ECE-1 antibody
Principal name
ECE-1 antibody
Alternative names for ECE-1 antibody
ECE1, ECE, Endothelin-converting enzyme 1
Ncbi ID
9606, NP_001106818, NP_001106818.1, NP_001388, NP_001106820, NP_955011.1, NM_199307, NM_001113348, NM_001113347, NM_001113349
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of ECE-1 antibody
- Kit Component:
- - KN304973G1, Ece1 gRNA vector 1 in pCas-Guide vector
- KN304973G2, Ece1 gRNA vector 2 in pCas-Guide vector
- KN304973D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of ECE-1 Antibody (Center) (AP17300PU-N) in Y79, T47D cell line and mouse lung, liver lysates (35 µg/lane). ECE-1 (arrow) was detected using the purified Pab.
Western blot analysis of ECE1 Antibody (Center) (AP17997PU-N) in HepG2 cell line lysates (35ug/lane). ECE1 (arrow) was detected using the purified Pab.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECE1 (RC223135, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECE1.
Predicted band size: 87 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue tissue using ECE1 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using ECE1 antibody.
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse lung tissue, Mouse liver tissue, Primary antibody: (ECE1 Antibody) at dilution 1/1050, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (ECE1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (ECE1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP17300PU-N | ECE-1 antibody |
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Rabbit | Ig | Aff - Purified | Bov, Hu, Ms, Rt | WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||