ABCF1 antibody
Principal name
ABCF1 antibody
Alternative names for ABCF1 antibody
ABC50, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein
Ncbi ID
9606, NP_001103353.1, NP_001020262, NP_001020262.1, NP_038882.1, NM_013854, NM_001109883, NM_001025091
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of ABCF1 antibody
- Kit Component:
- - KN300636G1, Abcf1 gRNA vector 1 in pCas-Guide vector
- KN300636G2, Abcf1 gRNA vector 2 in pCas-Guide vector
- KN300636D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of purified MaxPab antibody to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ABCF1 expression in transfected 293T cell line (H00000023-T01) by ABCF1 MaxPab polyclonal antibody.Lane 1: ABCF1 transfected lysate(92.95 KDa).Lane 2: Non-transfected lysate.
ABCF1 MaxPab polyclonal antibody. Western Blot analysis of ABCF1 expression in HepG2.
ABCF1 MaxPab polyclonal antibody. Western Blot analysis of ABCF1 expression in HeLa.
Immunofluorescence of purified MaxPab antibody to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ABCF1 expression in transfected 293T cell line (H00000023-T01) by ABCF1 MaxPab polyclonal antibody.Lane 1: ABCF1 transfected lysate(92.95 KDa).Lane 2: Non-transfected lysate.
ABCF1 MaxPab polyclonal antibody. Western Blot analysis of ABCF1 expression in HepG2.
ABCF1 MaxPab polyclonal antibody. Western Blot analysis of ABCF1 expression in HeLa.
Mouse Brain; WB Suggested Anti-Abcf1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Mouse Brain; Abcf1 antibody - C-terminal region (ARP43631_P050) in Mouse Brain cells using Western Blot
Hek293; Researcher: Dr. Joanna Stewart, Centre for Biological Sciences, University of Southampton. Application: IP. Species+tissue/cell type: 1: 500 ug HEK293 cell lysate transfected with ABCF1 wt, 2: 500 ug HEK293 cell lysate untransfected, 3: 500 ug HEK293 cell lysate transfected with mutABCF1Amount of IP antibody: 1ug. Primary antibody dilution: 1:1000. Secondary antibody: Anti-rabbit-Alexa Fluor 680. Secondary antibody dilution:1:20000 - See more at: http://www.avivasysbio.com/anti-abcf1-antibody-c-terminal-region-arp43631-p050.html#sthash.NAnGKqRc.dpuf; Abcf1 antibody - C-terminal region (ARP43631_P050) in Hek293 cells using Immunoprecipitation
ABCF1 Antibody (C-term) (Cat. #AP50022PU-N) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of ABCF1 Antibody (C-term)?Cat. #AP50022PU-N) in NCI-H460 cell line lysates (35µg/lane). ABCF1 (arrow) was detected using the purified Pab.
Western blot analysis of ABCF1 Antibody (C-term) (Cat. #AP50022PU-N) in mouse NIH-3T3 cell line lysates (35 µg/lane). ABCF1 (arrow) was detected using the purified Pab.
Western blot analysis of extracts from HeLa/COLO205 cells, using ABCF1 antibody.The lane on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ABCF1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using ABCF1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human gastric and liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ABCF1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human gastric and liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using ABCF1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis on HeLa cell lysate using ABCF1 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP50022PU-N | ABCF1 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
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