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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT1 / PKB pSer473 antibody

  Mouse monoclonal Akt phospho S473 antibody  
WB of Mouse Anti-AKT pS473 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4° C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:20,000 dilution. Mouse IgG1 17F6.B11 Purified Chimp, Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / $515.00
  OriGene Technologies, Inc.

CD339 / JAG1 (110-125) antibody

  Rabbit polyclonal anti-Jagged 1 antibody  
WB using anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).  Lane 4 contained sample buffer only.Each lane contains approximately 20 µg of lysate.  Primary antibody was used at a 1:500 dilution. Predicted molecular weight is 134 kDa. Rabbit IgG Aff - Purified Chimp, Hu, Ms, Rt E, ICC/IF, P, WB
0.5 mg / $590.00
  OriGene Technologies, Inc.

GLI3 (41-57) antibody

  Rabbit polyclonal anti-Gli-3 antibody  
WB of Rabbit anti-Gli-3 antibody. Lane 1: human brain whole cell lysate. Lane 2: human lung whole cell lysate. Lane 3: human spleen whole cell lysate. Lane 4: mouse brain whole cell lysate. Lane 5: mouse lung whole cell lysate. Load: 20 µg per lane. Primary antibody: Gli-3 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Mky, Ms, Rt, Xen E, P, WB
0.1 mg / $515.00
  OriGene Technologies, Inc.

HDAC1 (466-482) antibody

  Rabbit polyclonal anti-HDAC-1 antibody  
Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55kDa for HDAC-1. Other band(s): none. Rabbit IgG Aff - Purified Bov, Chimp, Chk, Hu, Ms, Rt E, P, WB
0.1 mg / $515.00
  OriGene Technologies, Inc.

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