TRIM2 / RNF86 antibody
Principal name
TRIM2 / RNF86 antibody
Alternative names for TRIM2 / RNF86 antibody
KIAA0517, RING finger protein 86, Tripartite motif-containing protein 2
Ncbi ID
9606, NP_001123539, ADZ16100.1, NP_001123539.1, NM_001130067, NP_109631.1, NM_030706, NM_015271
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of TRIM2 / RNF86 antibody
- Kit Component:
- - KN318189G1, Trim2 gRNA vector 1 in pCas-Guide vector
- KN318189G2, Trim2 gRNA vector 2 in pCas-Guide vector
- KN318189D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
TRIM2 polyclonal antibody ( Cat # PAB7498 ) ( 0.5 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP31074PU-N TRIM2 antibody staining of Rat Brain lysate at 0.5 mg/ml (35 µg protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody(TA501551),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody(TA501552),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TRIM2 antibody(TA501552),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-TRIM2 mouse monoclonal antibody (TA501553) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM2(RC203947).
Immunofluorescent staining of HeLa cells using anti-TRIM2 mouse monoclonal antibody (TA501553).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA501559), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA501560), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA501606), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-TRIM2 antibody(TA501606),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody(TA501606),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC203947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
HEK293T cells transfected with either RC203947 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM2 antibody(TA502192), and then analyzed by flow cytometry.
TA305745 (0.5ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501606 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) | |||||||
 |
Mouse | IgG1 | OTI2B10 | Purified | Hu | F, WB |
0.1 ml /
$379.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA305745 | TRIM2 / RNF86 (C-term) antibody |
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| Goat Anti-TRIM2 Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, IP, P, WB |
0.1 mg /
$360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA501551 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5) | |||||||
 |
Mouse | IgG2b | OTI4E5 | Purified | Hu, Rt | F, WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA501552 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI2B2 (formerly 2B2) | |||||||
 |
Mouse | IgG1 | OTI2B2 | Purified | Hu | F, WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA501553 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) | |||||||
 |
Mouse | IgG1 | OTI3B5 | Purified | Hu | ICC/IF, WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA501558 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI5G7 (formerly 5G7) | |||||||
 |
Mouse | IgG2b | OTI5G7 | Purified | Hu | WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA501559 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI1D2 (formerly 1D2) | |||||||
 |
Mouse | IgG1 | OTI1D2 | Purified | Hu | F, WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA501560 | TRIM2 / RNF86 antibody |
||||||
| TRIM2 mouse monoclonal antibody, clone OTI1D12 (formerly 1D12) | |||||||
 |
Mouse | IgG1 | OTI1D12 | Purified | Hu | F, WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA502192 | TRIM2 / RNF86 antibody |
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| TRIM2 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6) | |||||||
 |
Mouse | IgG1 | OTI4D6 | Purified | Hu | F, WB |
0.1 ml /
$350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP31074PU-N | TRIM2 / RNF86 (758-771) antibody |
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 |
Goat | Aff - Purified | Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Rb, Rt, Xen, Ze | E, P, WB |
50 µg /
$330.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN318189 | Trim2 - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318189BN | Trim2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318189LP | Trim2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
| KN318189RB | Trim2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY427142 | TRIM2 overexpression lysate |
|
|
0.1 mg /
$495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC203947L2V | Lenti ORF particles, TRIM2 (mGFP-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,731.00
|
|
| OriGene Technologies, Inc. | ||
| RC229368L2V | Lenti ORF particles, TRIM2 (mGFP-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,731.00
|
|
| OriGene Technologies, Inc. | ||
| RC226128L2V | Lenti ORF particles, TRIM2 (mGFP-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,731.00
|
|
| OriGene Technologies, Inc. | ||
| RC203947L1V | Lenti ORF particles, TRIM2 (Myc-DDK-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,556.00
|
|
| OriGene Technologies, Inc. | ||
| RC229368L1V | Lenti ORF particles, TRIM2 (Myc-DDK-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,556.00
|
|
| OriGene Technologies, Inc. | ||