SH2D2A antibody
Principal name
SH2D2A antibody
Alternative names for SH2D2A antibody
SCAP, TSAD, VRAP, SH2 domain-containing protein 2A, SH2 domain-containing adapter protein, T cell-specific adapter protein, VEGF receptor-associated protein
Ncbi ID
9606, NP_003966, NP_001154914.1, AAH12107, NM_003975, NM_001161442, NM_001161444, NM_001025571, NM_001161441, NM_001161443
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SH2D2A antibody
This gene encodes an adaptor protein thought to function in T-cell sigl transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kise and functions in downstream sigling. Altertive splicing results in multiple transcript variants. [provided by RefSeq].
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T01) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T01) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T02) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T03) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.90 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of SH2D2A transfected lysate using anti-SH2D2A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SH2D2A MaxPab mouse polyclonal antibody (B02) (H00009047-B02).
HEK293T cells were transfected with SH2D2A cDNA. After 48 hours, the cells were detached and fixed with 2% formaldehyde for 30 min, then permeabilized with 0.1% trixon-X100. The cells were then stained with Dylight488 conjugated mouse IgG1 (black line) or Dylight488 conjugated SH2D2A antibodies (red line).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Figure from citation: Comparison of IHC on tonsil with clone 4F3(TA504364) and clone 3C7(TA504372) SH2D2A mAb and HRP-conjugated secondary antibodies. View Citation
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Anti-SH2D2A mouse monoclonal antibody (TA504364) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A(RC204162).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504364), and then analyzed by flow cytometry.
Figure from citation: Comparison of IHC on tonsil with clone 4F3(TA504364) and clone 3C7(TA504372) SH2D2A mAb and HRP-conjugated secondary antibodies. View Citation
Anti-SH2D2A mouse monoclonal antibody (TA504372) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A(RC204162).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SH2D2A monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SH2D2A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504372), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504381), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504461), and then analyzed by flow cytometry.
Western blot analysis of extracts from HepG2 cells, using SH2D2A antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504364 | SH2D2A antibody |
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| SH2D2A mouse monoclonal antibody, clone OTI4F3 (formerly 4F3) | |||||||
 |
Mouse | IgG1 | OTI4F3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
 +11 additional images |
Published recently! Highly validated antibody!
|
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| TA504372 | SH2D2A antibody |
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| SH2D2A mouse monoclonal antibody, clone OTI3C7 (formerly 3C7) | |||||||
 |
Mouse | IgG1 | OTI3C7 | Purified | Hu, Mky, Ms | F, ICC/IF, P, WB |
0.1 ml /
$379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Published recently! Highly validated antibody!
|
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