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DPYSL2 / CRMP2 antibody

Principal name

DPYSL2 / CRMP2 antibody

Alternative names for DPYSL2 / CRMP2 antibody

Dihydropyrimidinase-related protein 2, DRP-2, N2A3, Collapsin response mediator protein 2

SwissProt ID

O02675 (Bovin), O08553 (Mouse), P47942 (Rat), Q16555 (Human), Q90635 (Chick)

Gene ID

1808 (DPYSL2), 12934 (Dpysl2), 25416

Ncbi ID

NP_001184222.1, 9606, NP_001184222, NP_001377, NP_034085.2, NM_009955, NM_001386, NM_001197293, NM_001105717

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C)

Background of DPYSL2 / CRMP2 antibody

DRP-2, also known as collapsin response mediator protein-2 (CRMP-2), is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

General readings

1. Greene ND, Bamidele A, Choy M, de Castro SC, Wait R, Leung KY, et al. Proteome changes associated with hippocampal MRI abnormalities in the lithium pilocarpine-induced model of convulsive status epilepticus. Proteomics. 2007 Apr;7(8):1336-44. PubMed PMID: 17366478.
2. Veyrac A. et al. Expression of collapsin response mediator proteins 1, 2 and 5 is differentially regulated in newly generated and mature neurons of the adult olfactory system. Eur J Neurosci. 2005;21:2635-48.
3. Bertin S. et al. Differential expression of CRMP1, CRMP2A, CRMP2B, and CRMP5 in axons or dendrites of distinct neurons in the mouse brain. J Comp Neurol. 2005;486:1-17.
4. Quach T. et al. Involvement of collapsin response mediator proteins in the neurite extension induced by neurotrophins in dorsal root ganglion neurons. Mol Cell Neurosci. 2004;25:433-43.
5. Charrier E. et al. Collapsin response mediator proteins (CRMPs): involvement in nervous system
development and adult neurodegenerative disorders. Mol Neurobiol. 2003;28:51-64. Review.
6. Ricard D et al. Isolation and expression pattern of human Unc-33-like phosphoprotein 6/collapsin
response mediator protein 5 (Ulip6/CRMP5): coexistence with Ulip2/CRMP2 in Sema3a- sensitive oligodendrocytes. J Neurosci. 2001 ; 21:7203-14.
7. Honnorat J. et al. Ulip/CRMP proteins are recognized by autoantibodies in paraneoplastic neurological syndromes. Eur J Neurosci. 1999;11:4226-32.

Immunohistochemistry analysis of paraffin-embedded human brain, using DRP-2 (Phospho-Thr514) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using DRP-2 (Phospho-Thr514) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30', using DRP-2 (Phospho-Thr514) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using CRMP-2 (Phospho-Thr509) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HT29 cells treated with heat shock, using CRMP-2 (Phospho-Thr509) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry: AM11031SU-N DPYSL2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AM11031SU-N

Immunohistochemistry: AM11031SU-N DPYSL2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human kidney followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AM11031SU-N

Western blot analysis of anti-DHP Monoclonal Antibody (Cat.#AM11031SU-N) by NIH-3T3 cell line lysates. DHP protein (arrow) was detected using the ascites Mab.

AM11031SU-N

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31330PU-N

Human Pancreas, Peripheral Nerve: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31330PU-N

Human Colon, Peripheral Nerve: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31330PU-N

Immunofluorescence analysis of HeLa cells, using DPYSL2 Antibody. The picture on the right is treated with the synthesized peptide.

AP31330PU-N

Western blot analysis of extracts from A549 cells, treated with EGF 200ng/ml 30', using DPYSL2 antibody. The lane on the right is treated with the synthesized peptide.

AP31330PU-N

mouse brain extract; WB Suggested Anti-DPYSL2 Antibody . . Positive Control: lane 1: 80ug mouse brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience. Secondry Antibody Dilution : 1:20,000. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science; DPYSL2 antibody - middle region (ARP51959_P050) in mouse brain extract cells using Western Blot

ARP51959_P050

Human brain; WB Suggested Anti-DPYSL2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human brain; DPYSL2 antibody - middle region (ARP51959_P050) in Human brain cells using Western Blot

ARP51959_P050

Rat brain; . Amount and Sample Type :. 500 ug rat brain homogenate. Amount of IP Antibody :. 6 ug. Primary Antibody :. DPYSL2. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:5000. Gene Name :. DPYSL2. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; DPYSL2 antibody - middle region (ARP51959_P050) in Rat brain cells using Immunoprecipitation

ARP51959_P050

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DRP-2 (Ab-514) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using DRP-2 (Ab-514) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from A549 cells, treated with EGF 200ng/ml 30', using DRP-2 (Ab-514) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CRMP-2 (Ab-509) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HT-29 cells, treated with heat shock, using CRMP-2 (Ab-509) Antibody. The lane on the right is treated with the synthesized peptide.

Distribution patternof CRMP2 in olfactory receptor neurons within the olfactory mucosa

AP54858SU-N

Immunoblot analysis of the specificity of CRMP2 antibody Cat.-No AP54858SU-N using E.coli lysate transformed with cDNA of Human CRMP2

AP54858SU-N

Immunohistochemical analysis of paraffin-embedded human brain tissue using CRMP-2 (Phospho-Thr509) antibody AP55813PU-N (left)or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from HT-29 cells treated with heat shock using CRMP-2 (Phospho-Thr509) Antibody AP55813PU-N.The lane on the right is treated with the antigen-specific peptide.

Western blot analysis of extracts from HuvEc cells (Lane 2), using CRMP-2 (Phospho-Ser522) Antibody AP55854PU-N. The lane on the left is treated with antigen-specific peptide.

Western blot analysis of DRP-2 expression in EGF treated A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325418

Western blot analysis of extracts from HT-29 cells, treated with heat shock, using CRMP-2 (Ab-509) antibody.The lane on the right is treated with the synthesized peptide.

TA313763

Western blot analysis of extracts from COS7 cells (Lane 2), using CRMP-2 (Ab-509) Antibody. The lane on the left is treated with systhesized peptide.

TA313763

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CRMP-2 (Ab-509) antibody.The picture on the right is treated with the synthesized peptide.

TA313763

Western blot analysis of extracts from HepG2 cells, Jurkatcells and 293 cells, using CRMP-2 (Ab-522)antibody.The lane on the right is treated with the synthesized peptide.

TA313765

Western blot analysis of extracts from HuvEc cells (Lane 2) and JK cells (Lane 3), using CRMP-2 (Ab-522) Antibody. The lane on the left is treated with systhesized peptide.

TA313765

Western blot analysis of extracts from A549 cells, treated with EGF (200ng/ml, 30mins), using DRP-2 (Ab-514) antibody.The lane on the right is treated with the synthesized peptide.

TA313767

Immunohistochemistry analysis of paraffin-embedded human braIin tissue using DRP-2 (Ab-514) antibody.The picture on the right is treated with the synthesized peptide.

TA313767

Immunofluorescence analysis of HeLa cells, using DRP-2 (Ab-514) antibody.The picture on the right is treated with the synthesized peptide.

TA313767

Predicted band size: 62kDa. Positive control: NIH/3T3 cell lysate . Recommended dilution: 1/200-1000

TA323185

Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue. Recommended dilution: 1/50-300 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using DPYSL2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA323186

Western blot analysis of DRP-2 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325419

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP31330PU-N	DPYSL2 / CRMP2 (C-term) antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	50 µl / $425.00
						OriGene Technologies GmbH
+4 additional images	Highly validated antibody!

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