Alpha-Synuclein / SNCA antibody

Principal name

Alpha-Synuclein / SNCA antibody

Alternative names for Alpha-Synuclein / SNCA antibody

NACP, PARK1, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

SwissProt ID

O55042 (Mouse), P37377 (Rat), P37840 (Human), Q3T0G8 (Bovin)

Gene ID

6622 (SNCA), 29219 (Snca), 20617

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Chk (Chicken), Sh (Sheep), Rb (Rabbit), Mam (Mammalia)

Available hosts

Mouse, Rabbit, Goat, Sheep, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Radioimmunoassay (R), EPair for Elisa (EPair), Dot blot (Dot), Immunoelectron Microscopy (EM)

Background of Alpha-Synuclein / SNCA antibody

Alpha-synuclein is expressed principally in the central nervous system (brain) but is also expressed in low concentrations in a variety of tissues except liver. It is predominantly expressed in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum of the CNS. It is primarily a neuronal protein, but can also be found in the neuroglial cells. It is concentrated in presynaptic nerve terminals of neurons, as well as having reported nuclear and mitochondrial localization. Alpha-synuclein interacts with plasma membrane phospholipids. Alpha-synuclein in solution is considered to be an intrinsically disordered protein and thus lacks a stable secondary or tertiary structure. However, data suggests the presence of partial alpha helical as well as beta sheet structures as well as mostly structured tetrameric states in solution, the equilibrium of which may be altered by binding partners. The human alpha-synuclein protein is made of 140 amino acids, encoded by the SNCA gene. The primary structure is divided in three distinct domains: (1-60) - An amphipathic N-terminal region dominated by four 11-residue repeats including the consensus sequence KTKEGV. This sequence has a structural alpha helix propensity similar to apolipoproteins-binding domains. (61-95) - a central hydrophobic region which includes the non-amyloid- beta; component (NAC) region, involved in protein aggregation. (96-140) - a highly acidic and proline-rich region. At least three isoforms of synuclein are produced through alternative splicing. The most common form of the protein, is the full 140 amino acid-long transcript. Other isoforms are alpha-synuclein-126, lacking residues 41-54; and alpha-synuclein-112, which lacks residues 103-130. a-synuclein may be involved in the regulation of dopamine release and transport and also may function to induce fibrillization of microtubule-associated protein tau. alpha-synuclein functions as a molecular chaperone in the formation of SNARE complexes. In particular, it can bind to phospholipids of the plasma membrane and to synaptobrevin-2 via its C-terminus domain to influence synaptic activity. alpha-synuclein is essential for normal development of the cognitive functions and that it significantly interacts with tubulin. It also reduces neuronal responsiveness to various apoptotic stimuli, leading to decreased caspase-3 activation. Alpha-synuclein fibrils are major substituent of the intracellular Lewy bodies seen in Parkinson rsquo;s disease. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.

General readings

1. Jakes, R., et al. (1994) FEBS lett. 345:27-32.
2. Ueda, K., et al. (1993) Proc. Natl.Acad. Sci. USA 90:11282-11286.
3. Kim, J. (1997) Molecules and Cells 7:78-83.
4. Paik, S. R., et al. (1997) Arch. Biochem. Biophys. 344:325-334.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-Synuclein / SNCA (also beta reactive) antibody

Flow cytometry analysis of α,β -Synuclein LNCaP cell line staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG mouse IgG Alexa fluor 488 conjugate Isotype control antibody was mouse IgG (black line). Mouse IgG1 3B6 Purified Hu, Ms, Rt E, F, ICC/IF, WB
0.1 ml / $350.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA (also beta reactive) antibody

Flow cytometry analysis of α,β -Synuclein LNCaP cell line staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG mouse IgG Alexa fluor 488 conjugate Isotype control antibody was mouse IgG (black line). Mouse IgG1 3B6 Purified Hu, Ms, Rt E, F, ICC/IF, WB
50 µl / $255.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

AP32035PU-N Alpha-Synuclein / SNCA Antibody staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / $330.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA (NACP140) antibody

AP32036PU-N Alpha-Synuclein / SNCA Antibody staining of Human Amygdala lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Por WB
0.1 mg / $330.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

  Mouse Anti-alpha Synuclein Antibody  
Western blot of a rat brain lysate showing specific immunolabeling of the ~15k alpha synuclein protein Mouse IgG1 3H9 Ascites Hu, Mam, Ms, Rt ICC/IF, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit Anti-Alpha synuclein (ser129) Antibody (Phospho-Specific)  
Western blot of rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129.  Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown). Rabbit IgG Purified Rt WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit polyclonal a-Synuclein (Ab-133) antibody  
Western blot analysis of extracts from 293 cells, treated with Etoposide 25µM 60', using Synuclein-alpha (Ab-133) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit polyclonal Synuclein-pan antibody  
Western blot analysis of extracts from rat heart, using Synuclein-pan antibody (#TA311770).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Anti-SNCA (Phospho-Tyr125) Rabbit Polyclonal Antibody  
Predicted band size: 18 kDa . Positive control: C2C12 cells treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: C2C12 cells untreated with Anisomycin lysate Lane2: C2C12 cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu ICC/IF, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Anti-SNCA (Phospho-Tyr136) Rabbit Polyclonal Antibody  
Predicted band size: 18 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: Treated with the peptide Lane2: Mouse brain tissue lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Anti-SNCA Rabbit Polyclonal Antibody  
Predicted band size: 14 kDa . Positive control: Mouse Brain tissue lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit Polyclonal Synuclein Antibody  
Western blot analysis of Synuclein expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit Polyclonal Synuclein-alpha Antibody  
Alpha-Synuclein / SNCA Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit Polyclonal Alpha-synuclein Antibody  
Western blot analysis on mouse brain cell lysate using Alpha-synuclein Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Mouse Monoclonal Synuclein-alpha Antibody (2A7)  
Western Blot: alpha-Synuclein Antibody (2A7) [TA336706] - Blots of TA336706 on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane, courtesy Rogan Tinsley, Howard Florey Institute, University of Melbourne). Mouse IgG1 2A7 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Mouse Monoclonal Synuclein-alpha [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8)  
Western Blot: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [TA336747] - Western blot of (A) Nitrated alpha-Synuclein protein and (B) non-nitrated alpha-Synuclein protein. Mouse IgG1 24.8 Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / $379.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Mouse Monoclonal Synuclein-alpha Antibody (3H9)  
Western Blot: alpha-Synuclein Antibody (3H9) [TA336858] - WB validation of TA336858 on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane) Mouse IgG1 3H9 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Chicken Polyclonal Synuclein-alpha Antibody  
Western Blot: alpha-Synuclein Antibody [TA337099] - Crude extract of rat brain, showing strong band at 15 kDa. Chicken Purified Rt ICC/IF, WB
0.1 ml / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit Polyclonal Anti-SNCA Antibody  
Western blot analysis of SNCA in mouse cerebellum tissue lysate with SNCA antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / $325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit Polyclonal anti-Synuclein-alpha Antibody  
Alpha-Synuclein / SNCA Rabbit Purified Hu P
0.1 ml / $325.00
  OriGene Technologies, Inc.

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