AR09676PU-L Deoxycytidine kinase (1-260, His-tag)

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0.5 mg / $1,020.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Deoxycytidine kinase

AR09676PU-L

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  • AR09676PU-L

Product Description for Deoxycytidine kinase

Deoxycytidine kinase.
Presentation: Purified

Properties for Deoxycytidine kinase

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms DCK, MGC117410, MGC138632
Presentation Purified
Molecular weight 34.6 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P27707
Purity > 90 %
Background DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5`-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or UTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Radu CG., et al. (2010) Proc Natl Acad Sci U S A. 107(12):5551-6.
Ribeiro R., et al. (2007) J Pharmacol Exp Ther. 323(3):935-45.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MADPWQECMD YAVTLARQAG EVVCEAIKNE MNVMLKSSPV DLVTATDQKV EKMLISSIKE KYPSHSFIGE ESVAAGEKSI LTDNPTWIID PIDGTTNFVH RFPFVAVSIG FAVNKKIEFG VVYSCVEGKM YTARKGKGAF CNGQKLQVSQ QEDITKSLLV TELGSSRTPE TVRMVLSNME KLFCIPVHGI RSVGTAAVNM CLVATGGADA YYEMGIHCWD VAGAGIIVTE AGGVLMDVTG GPFDLMSRRV IAANNRILAE RIAKEIQVIP LQRDDED
Molecular weight:
(296aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol
Purity:
>90%
State:
Liquid purified protein
Purified
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