Antibody Panel to proteins of the superoxide dismutase enzyme family (SOD1, SOD2, SOD3, SOD4) - FocusOn 112

disables radicals*   disables radicals

Superoxide dismutase (SOD) is an antioxidant enzyme and as such part of the defense system against reactive oxygen species (ROS). SOD catalyses the dismutation reaction of the superoxide radical anion (O2-) to hydrogen peroxide (H2O2) through glutathione reductase and catalase.

Several classes of SOD have been identified. These include the intracellular copper/zinc SOD (Cu/Zn-SOD, SOD-1), the mitochondrial manganese SOD (Mn-SOD, SOD2) and the extracellular copper/zinc SOD (ECSOD, SOD3).

SOD-1 is found in all eukaryotic species as homodimeric 32 kD enzyme, which does contain one Cu- and one Zn-ion each. SOD1 neutralizes supercharged oxygen molecules (superoxide radicals), which can damage cells if their levels are not controlled. The superoxide dismutase enzyme probably gains new, but harmful, properties when altered by SOD1 mutations. It is unclear why nerve cells in the brain and spinal cord that control muscle movement (motor neurons) are particularly sensitive to SOD1 mutations.

The Mn-containing, tetrameric enzyme SOD2 is localized at the mitochondrial matrix, in close proximity to the primary endogen source for superoxide, the respiratory chain. For this reason it is excellent as mitochondrial marker.

SOD3 is a heparin-binding multimer of dimers, which is expressed primarily in human lung, vessel walls and airways. It is the major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. Extracellular superoxide dismutase SOD3 (EC-SOD) protects the extracellular space from toxic effect of reactive oxygen intermediates.

SOD4 is a Cu-Chaperon for SOD (CCS), which specifically delivers Cu to SOD1. CCS presumably activates the Cu/Zn SOD by direct insertion of the Cu-cofactor.



Western blot analysis of tissue lysates with SOD-2 antibody LF-MA0030

Fig. 1: Western blot analysis of tissue lysates with SOD-2 antibody LF-MA0030
Lane 1: HeLa cell lysate
Lane 2: Mouse brain
Lane 3: Ratte brain



Detection and Analysis of SOD

Acris Antibodies offers a vast selection of antibodies, proteins and ELISA kits (see table below), which can be used to analyse the four isoforms of SOD in Western blots and other methods.



Literature

Eisen A, Mezei MM, Stewart HG, Fabros M, Gibson G, Andersen PM. SOD1 gene mutations in ALS patients from British Columbia, Canada: clinical features, neurophysiology and ethical issues in management. Amyotroph Lateral Scler. 2008 Apr; 9(2):108-19.

Proescher JB, Son M, Elliott JL, Culotta VC. Biological effects of CCS in the absence of SOD1 enzyme activation: implications for disease in a mouse model for ALS. Hum Mol Genet. 2008 Jun 15;17(12):1728-37

Available SOD Antibodies

SOD-1 antibody, SOD-2 antibody, SOD-3 antibody, SOD-4 antibody, SOD-1 protein, SOD-2 protein, SOD-3 protein, SOD-4 protein, SOD-1 ELISA Kit, SOD-2 ELISA Kit.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Superoxide Dismutase 1 / SOD1 antibody

Immunohistochemistry: AM01230PU-N SOD1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Liver at 15 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 2F5 Purified Bov, Hu, Ms C, E, P
0.1 mg / $400.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of SOD expression with Jurkat cell lysates (Lane 1, 2). Rabbit Aff - Purified Eq, GP, Hu, Ms, Rt C, IP, P, WB
0.1 mg / $290.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 (Center) antibody

Western blot analysis of SOD1 Antibody (Center) (AP18205PU-N) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / $330.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Superoxide Dismutase 1 / SOD1 Sheep IgG Purified Bov, Hu C, E
1 ml / $390.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Superoxide Dismutase 1 / SOD1 Rabbit IgG Serum Bov E, WB
2 ml / $1,070.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Superoxide Dismutase 1 / SOD1 Mouse IgG1 SOD1 Purified Hu E, WB
1 mg / $380.00
  HyTest Ltd.

Superoxide dismutase 2 / SOD2 antibody

Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOD2 Monoclonal Antibody (Cat.#AM11059PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Mouse IgG1 37CT127.5.11.6 Purified Hu P, WB
0.4 ml / $330.00
  Acris Antibodies GmbH

Superoxide dismutase 2 / SOD2 antibody

Superoxide dismutase 2 / SOD2 Mouse IgM SOD2 Purified Hu E, WB
1 mg / $380.00
  HyTest Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Superoxide Dismutase 1 / SOD1 (1-154)

Superoxide Dismutase 1 / SOD1 Human Purified > 95 % pure by SDS-PAGE E. coli
0.5 mg / $790.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 (1-154)

Superoxide Dismutase 1 / SOD1 Human Purified > 95 % pure by SDS-PAGE E. coli
0.1 mg / $340.00
  Acris Antibodies GmbH

Superoxide dismutase 2 / SOD2 (25-222, His-tag)

Superoxide dismutase 2 / SOD2 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / $695.00
  Acris Antibodies GmbH

Superoxide dismutase 2 / SOD2 (25-222, His-tag)

Superoxide dismutase 2 / SOD2 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / $305.00
  Acris Antibodies GmbH

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