Antibodies to Catenins - FocusOn 107

Introduction: Catenins are cytoplasmic linker proteins of cell-cell junctions

This protein family include the alpha-, beta-, and gamma-Catenins. In adherens junctions, alpha-Catenin links the Cadherin/beta-Catenin complex to the actin-based cytoskeleton via a direct association between alpha Actinin and alpha Catenin. Beta-Catenin was originally identified as a binding protein of Cadherin. Beta-Catenins associate with the cytoplasmic domain of the adhesion molecules N- and E-Cadherin and are essential for Cadherin function in cell-cell adhesion. It plays a dual role as structural component of cell-cell junctions and as regulatory protein in the nucleus. Beta-Catenin can transduce changes in cell adhesion and junction formation to control transmembrane signaling and gene expression. Delta Catenin is present in adhering type junctions in the nervous system, primarily expressed in the brain and is described to play a role in familial Alzheimer’s disease. Gamma Catenin (Plakoglobin) is found in desmosomes and adherens junctions (see Newsletter Summer 2007 for more detailed information).

Antibody Tools for the Detection of Catenins alpha, beta, gamma and delta

Table 1 shows Acris wide range of antibodies for detection of Catenin family members. Most of these anti-bodies are suitable for Western blots and immunohistochemistry. Please note, that some products do include a positive control for use in Western blots and that we also offer antibodies for detection of phosphorylated beta-Catenin.



Phosphospecificity. Whole cell extracts of control (1) or pervanadate treated (2) SW480 tumor cells were applied to SDS-PAGE and transferred to a PVDF

 

Fig.1: Phosphospecificity. Whole cell extracts of control (1) or pervanadate treated (2) SW480 tumor cells were applied to SDS-PAGE and transferred to a PVDF. The immunoblot was probed with beta-Catenin antibody (phosphoTyr86) cat. no. AM00015PU-N.



Literature:

Conacci-Sorrell, M; Zhurinsky, J; Ben-Ze'ev, A (2002) The cadherin-catenin adhesion system in signaling and cancer. J. Clin. Invest. 109:987-991.

Suzuki SC, Takeichi M. Cadherins in neuronal morphogenesis and function. Dev Growth Differ, 2008 Jun; 50 Suppl 1: 119-130.

Available Antibody Panel to Catenin protein family

All Catenin antibody products: Alpha Catenin antibody, beta Catenin antibody, gamma Catenin (Plakoglobin)) antibody, delta Catenin antibody.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Catenin alpha-1 / CTNNA1 (incl. pos. control) antibody

Detection of endogenous alpha catenin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab a-Cat-15D9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 15D9 Purified Can, Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using, Alpha-Catenin Antibody (#AP0876PU) and Alpha-Catenin (Phospho-Ser641) Antibody (#AP09467PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / $270.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using, Alpha-Catenin Antibody (#AP0876PU) and Alpha-Catenin (Phospho-Ser641) Antibody (#AP09467PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / $205.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 pSer641 antibody

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody (Cat.-No AP09467PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / $300.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 pSer641 antibody

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody (Cat.-No AP09467PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / $235.00
  Acris Antibodies GmbH

Catenin alpha-3 / CTNNA3 (164-177) antibody

Immunohistochemistry on Paraffin Section of Human heart. Mouse IgG2a 892_24D2S Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / $340.00
  Acris Antibodies GmbH

Catenin beta-1 (exon 3) (incl. pos. control) antibody

Detection of endogenous beta-Catenin: Whole cell lysates of serum starved tumor cells (20.000 cells/lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM00020PU-N (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431
Lane 2: A549
Lane 3: SKOV3
Lane 4: OVCAR5
Lane 5: HaCaT
Lane 6: PC3
Lane 7: HeLa
Lane 8: HepG2 Mouse IgG1 9G2 Purified Can, Hu, Ms E, ICC/IF, IP, P, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 pTyr86 (incl. pos. control) antibody

Catenin beta-1 Mouse IgG1 24E1 Biotin Hu, Ms, Rt E, WB
0.1 mg / $550.00
  Acris Antibodies GmbH

Catenin beta-1 pTyr86 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (1) or pervanadate treated (2) SW480 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and  transferred to a PVDF membrane. The immunoblot was probed with mab b-Cat-24E1 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 24E1 Purified Hu, Ms, Rt E, WB
0.1 mg / $480.00
  Acris Antibodies GmbH

Catenin beta-1 pTyr654 (incl. pos. control) antibody

Catenin beta-1 Mouse IgG1 1B11 Biotin Can, Hu, Ms WB
0.1 mg / $550.00
  Acris Antibodies GmbH

Catenin beta-1 pTyr654 (incl. pos. control) antibody

Whole cell extracts of control (1) or pervanadate treated (2)OVCAR 5 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with antibody AM00016PU-N (clone 1B11) at 0.5 μg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 1B11 Purified Can, Hu, Ms WB
0.1 mg / $480.00
  Acris Antibodies GmbH

Catenin beta-1 (dephospho 27-37) (incl. pos. control) antibody

Detection of endogenous β-Catenin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab b-Cat-8E4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D Mouse IgG1 8E4 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 (dephospho 35-50) (incl. pos. control) antibody

Detection of endogenous β-Catenin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab b-Cat-7A7(0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D Mouse IgG1 7A7 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 (N-term, exon 2) (incl. pos. control) antibody

Detection of endogenous β-Catenin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab b-Cat-7D11 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D Mouse IgG2a 7D11 Purified Hu, Ms, Rt E, IP, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 (exon 3) (incl. pos. control) antibody

Catenin beta-1 Mouse IgG1 9G2 Biotin Can, Hu, Ms E, ICC/IF, IP, P, WB
0.1 mg / $480.00
  Acris Antibodies GmbH

Catenin beta-1 (core) (incl. pos. control) antibody

Detection of endogenous b-catenin: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF  membranes. Immunoblots were probed with mab b-cat 9G10 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment : 15 min lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6:  MDA231; lane 7: T47D Mouse IgG2b 9G10 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 (C-term) (incl. pos. control) antibody

Detection of endogenous β-Catenin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab b-Cat-10H8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D Mouse IgG2a 10H8 Purified Hu, Ms, Rt E, IP, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 (C-term) (incl. pos. control) antibody

Detection of endogenous beta-Catenin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab b-Cat-7D8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min).
Lane 1: A431 
Lane 2: SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA-MB231 
Lane 7: T47D Mouse IgG1 7D8 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / $410.00
  Acris Antibodies GmbH

Catenin beta-1 pSer45 antibody

Catenin beta-1 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / $430.00
  Acris Antibodies GmbH

Catenin beta-1 pThr41/pSer45 antibody

Western blot (WB) analysis of p-Catenin-β (pThr41+S45) antibody Cat.-No.: AP01543PU-N in extracts from sw626 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / $330.00
  Acris Antibodies GmbH

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