Antibodies to B-cell Linker Protein (BLNK) - FocusOn 079
Linker or adapter proteins provide mechanisms by which receptors can amplify and regulate downstream effector proteins. BLNK (also referred to as BASH or SLP-65) is an adaptor molecule that plays a key role in B cell activation initiated by cross-linking the B cell receptor (BCR).
This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of non-receptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is a cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck. BLNK regulates B cell proliferation and differentiation; the recently reported complete loss or drastic reduction of BLNK expression in ~50% of human childhood pre-B acute lymphoblastic leukaemias (ALL) may be one example for its crucial function.
Antibody Panel to B-cell Linker Protein (BLNK)
Acris Antibodies offers a panel of monoclonal and polyclonal antibodies for the detection of BLNK in human, rat or mouse tissues. Antibodies can be used for Immunohistochemistry, Western blotting or other Immunological methods.
Antibody Panel to BLNK
B-cell Linker Protein (BLNK), BASH, SLP-65, antibody.
Xu S, Huo J, Chew WK, Hikida M, Kurosaki T, Lam KP. Phospholipase Cgamma2 dosage is critical for B cell development in the absence of adaptor protein BLNK. J Immunol. 2006 Apr 15;176(8):4690-8.
Gupta N, Delrow J, Drawid A, Sengupta AM, Fan G, Gélinas C. Repression of B-cell linker (BLNK) and B-cell adaptor for phosphoinositide 3-kinase (BCAP) is important for lymphocyte transformation by rel proteins. Cancer Res. 2008 Feb 1;68(3):808-14.
Chui, C.W., et al. (2002) BLNK: molecular scaffolding through ‘cis’-mediated organization of signaling proteins. EMBO J. 23:6461-6472 (cites the use of this antibody in Western blotting).
Kabak, S., et al. (2002) The direct recruitment of BLNK to immunoglobulin alpha couples the B-cell antigen receptor to distal signaling pathways. Mol. Cell. Biol. 22(8):2524-2535.
Taguchi, T. et al. (2004) Deficiency of BLNK hampers PLC-gamma 2 phosphorylation and Ca2+ influx induced by the pre-B cell receptor in human pre-B cells.Immunology 112(4): 575-582.
- Highly Validated (1)
|Mouse||IgG1||5G9||Ascites||Hu, Ms||E, F, ICC/IF, P, WB||
0.1 ml / $360.00
|Acris Antibodies GmbH|
|+5 additional images||
Highly validated antibody!
0.1 mg / $255.00
|Acris Antibodies GmbH|