BM6008HRP Vimentin antibody

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1 ml / $460.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Mouse anti Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Zebrafish Vimentin RV202

BM6008HRP

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Product Description for Vimentin

Mouse anti Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Zebrafish Vimentin RV202.
Presentation: HRP
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Vimentin

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms VIM
Presentation HRP
Reactivity Can, Chk, Gt, Hst, Hu, Mky, Ms, Por, Rt, Ze
Applications C, F, ICC/IF
Clonality Monoclonal
Clone RV202
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08670
Immunogen:
Cytoskeletal Vimentin extract of Bovine lens.
GeneID:
7431
Application Immunoblotting. 
Flow Cytometry.
Immunocytochemistry (cell cultures and cytospins).
Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Recommended Dilutions: 1/10.
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
General Readings
  1. Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P. Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res. 1987 May;170(1):235-49. PubMed PMID: 2436934.
  2. Viebahn C, Lane EB, Ramaekers FC. Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res. 1988 Sep;253(3):553-62. PubMed PMID: 2460241.
  3. Pieper FR, Schaart G, Krimpenfort PJ, Henderik JB, Moshage HJ, van de Kemp A, et al. Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol. 1989 Mar;108(3):1009-24. PubMed PMID: 2646305. (Free PMC Article available)
  4. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990 Nov;111(5 Pt 1):1971-85. PubMed PMID: 1699950. (Free PMC Article available)
  5. Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641-55. PubMed PMID: 1690512. (Free PMC Article available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
HRP
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.1% BSA
State:
Liquid IgG fraction
Species Reactivity
Species reactivity (tested):
Canine, Chicken, Caprine, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish, Xenopus.
Specificity
Specificity:
RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

Accessory Products

Proteins and/or Positive Controls

Proteins for Vimentin (10 products)

Catalog No. Species Pres. Purity   Source  

Vimentin

Vimentin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Vimentin

Vimentin Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / $135.00
  OriGene Technologies, Inc.

Vimentin (1-466)

Vimentin Human Aff - Purified > 95 % (by SDS-PAGE) E. coli
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified > 95 % pure determined by SDS Gel Electrophoresis E. coli
0.25 mg / $580.00
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified > 95 % pure determined by SDS Gel Electrophoresis E. coli
0.1 mg / $390.00
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified Greater than 95.0% (SDS-gelelctrophoresis) E. coli
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified Greater than 95.0% (SDS-gelelctrophoresis) E. coli
  OriGene Technologies GmbH

Vimentin

Vimentin Human Purified Greater than 95.0% (SDS-gelelctrophoresis) E. coli
  OriGene Technologies GmbH
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