TA319239 SMAD1 antibody

Rabbit polyclonal anti-SMAD1 antibody

See related secondary antibodies

Search for all "SMAD1"

0.1 mg / $515.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Canine, Chimpanzee, Chicken, Drosophila, Equine, Human, Macaque, Mouse, Opossum, Orang-Utan, Porcine, Rat, Sheep SMAD1

Product Description for SMAD1

Rabbit anti Canine, Chimpanzee, Chicken, Drosophila, Equine, Human, Macaque, Mouse, Opossum, Orang-Utan, Porcine, Rat, Sheep SMAD1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for SMAD1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms BSP-1, BSP1, JV4-1, MAD homolog 1, MADH1, MADR1, Mad-related protein 1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD 1, SMAD family member 1, SMAD-1, Transforming growth factor-beta-signaling protein 1
Presentation Aff - Purified
Reactivity Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q15797
Immunogen:
Synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein
GeneID:
4086
Application ELISA: 1/25,000 - 1/30,000.
Western Blot: 1/500.
Background SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro; this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasomemediated degradation.
Concentration 1.31 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Muqit, M & Alessi, D. (2007) Identification of substrates and regulators of the Parkinson’s disease associated protein kinase, PINK1. Mol Cell. Feb 9; 25:441-454.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Storage Conditions for Trial Size:
This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Shelf life: 3 month from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
State:
Liquid purified Ig
Aff - Purified
Species Reactivity
Species reactivity (expected):
Duckbill platypus, Drosophila, Mouse, Opossum, Chimpanzee, Bonobo, Orangutan, Gorilla, Macaque, Cattle, Sheep, Porcine, Rat, Horse, Salmon, Chicken, Canine.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is directed against SMAD1 protein.

Accessory Products

Proteins and/or Positive Controls

Proteins for SMAD1 (3 products)

Catalog No. Species Pres. Purity   Source  

SMAD1 (transcript variant 2)

SMAD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

SMAD1 (transcript variant 2)

SMAD1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / $299.00
  OriGene Technologies, Inc.

SMAD1 (full length, N-term HIS tag, transcript variant 1)

SMAD1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / $205.00
  OriGene Technologies, Inc.

Positive controls for SMAD1 (1 products)

Catalog No. Species Pres. Purity   Source  

SMAD1 overexpression lysate

SMAD1 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.
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