SM6006S SETD7 / SET9 (1-366) antibody

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50 µl / $255.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Mouse anti Human SETD7 / SET9 s4E5

SM6006S

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Product Description for SETD7 / SET9

Mouse anti Human SETD7 / SET9 s4E5.
Properties: (1-366)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for SETD7 / SET9

Product Category Primary Antibodies
Target Category
Quantity 50 µl
Synonyms H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7, SET7/9
Presentation Purified
Reactivity Hu
Applications E, F, ICC/IF, P, WB
Clonality Monoclonal
Clone s4E5
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8WTS6
Immunogen:
Recombinant Human SET7/9 protein (1-366aa) purified from E. coli
GeneID:
80854
Property 1-366
Isotype control AM03110PU-N, SM12P
Add. information Predicted Molecular Weight: 50 kDa.
Application ELISA.
Western blot: 
1/500-1/2,000. 
Recommended Starting Dilution: 1/1,000.
Immunohistochemistry on Paraffin Sections: 5 µg/ml after heat induced antigen retrieval in pH 6.0 citrate buffer.
Immunocytochemistry / Immunoflourescence.
Flow cytometry.

Background Set7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression.
Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.
Concentration 1.0 mg/ml
General Readings
  1.  Protein demethylation required for DNA methylation. Hotz HR, Peters AH.Nat Genet. 2009 Jan;41(1):10-1.
  2. Xiao B, Jing C, Wilson JR, Walker PA, Vasisht N, Kelly G, et al. Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature. 2003 Feb 6;421(6923):652-6. Epub 2003 Jan 22. PubMed PMID: 12540855.
  3. Kwon T, Chang JH, Kwak E, Lee CW, Joachimiak A, Kim YC, et al. Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet. EMBO J. 2003 Jan 15;22(2):292-303. PubMed PMID: 12514135. (Free PMC Article available, 5 images available)
  4. Nishioka K, Chuikov S, Sarma K, Erdjument-Bromage H, Allis CD, Tempst P, et al. Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation. Genes Dev. 2002 Feb 15;16(4):479-89. PubMed PMID: 11850410. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
The antibody recognizes Human SET7/9.
Other species not tested.
Gene ID 80854
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for SETD7 / SET9 (3 products)

Catalog No. Species Pres. Purity   Source  

SETD7 / SET9

SETD7 / SET9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $680.00
  OriGene Technologies, Inc.

SETD7 / SET9

SETD7 / SET9 Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $270.00
  OriGene Technologies GmbH

SETD7 / SET9

SETD7 / SET9 Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $585.00
  OriGene Technologies GmbH

Positive controls for SETD7 / SET9 (1 products)

Catalog No. Species Pres. Purity   Source  

SETD7 overexpression lysate

SETD7 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.
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