AP12693PU-N RBL1 pSer975 antibody

See related secondary antibodies

Search for all "RBL1"

0.4 ml / $330.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human RBL1

AP12693PU-N

More Views

  • AP12693PU-N

Product Description for RBL1

Rabbit anti Human RBL1.
Properties: pSer975
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for RBL1

Product Category Primary Antibodies
Target Category
Quantity 0.4 ml
Synonyms 107 kDa retinoblastoma-associated protein, Retinoblastoma-like protein 1, p107, pRb1
Presentation Aff - Purified
Reactivity Hu
Applications P, WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P28749
Immunogen:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like 1.
GeneID:
5933
Property pSer975
Add. information Molecular weight: 120877 Da
Application ELISA: 1/1,000.
Western Blot: 1/100-1/500.
Immunohistochemistry: 1/50~1/100.
Background RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.
Caution This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Concentration lot specific
General Readings
  1. Rodier, G., et al., J. Cell Biol. 168(1):55-66 (2005).
  2. Barbie, T.U., et al., Proc. Natl. Acad. Sci. U.S.A. 100(26):15601-15606 (2003).
  3. Joaquin, M., et al., J. Biol. Chem. 278(45):44255-44264 (2003).
  4. Cicchillitti, L., et al., J. Biol. Chem. 278(21):19509-19517 (2003).
  5. Leng, X., et al., Mol. Cell. Biol. 22(7):2242-2254 (2002).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Buffer System:
PBS with 0.09% (W/V) Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects Rb-like-1 pSer975.
Species:
Human.
Gene ID 5933

Accessory Products

Proteins and/or Positive Controls

Positive controls for RBL1 (1 products)

Catalog No. Species Pres. Purity   Source  

RBL1 overexpression lysate

RBL1 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.
  • LinkedIn