AP31995PU-N PRDM16 (1137-1150) antibody

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Goat anti Canine, Mouse, Rat PRDM16

Product Description for PRDM16

Goat anti Canine, Mouse, Rat PRDM16.
Properties: (1137-1150)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for PRDM16

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms KIAA1675, MDS1/EVI1-like gene 1, MEL1, PFM13, PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1
Presentation Aff - Purified
Reactivity Can, Ms, Rt
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Synthetic peptide with sequence from the internal region (near C-Terminus) of Mouse Prdm16 (NP_081780.3; NP_001171466.1)
AA Sequence:
Property 1137-1150
Application Peptide ELISA: 1/8000 (Detection Limit).
Western blot: 1-3 µg/ml. Detects a band of Approx 150kDa in Mouse Brain lysates (calculated MW of 141kDa according to NP_081780.3).
An additional band of unknown identity was also consistently observed at 85kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
Concentration 0.5 mg/ml

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
0.02% Sodium Azide
0.5% BSA
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Rat, Canine (Dog).
Species reactivity (tested):
This antibody is expected to recognize both reported isoforms of Prdm16 (NP_081780.3; NP_001171466.1).

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