AP60005PU-N PRAME / MAPE (C-term) antibody

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0.1 mg / $270.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Rabbit anti Human PRAME / MAPE

AP60005PU-N

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  • AP60005PU-N

Product Description for PRAME / MAPE

Rabbit anti Human PRAME / MAPE.
Properties: (C-term)
Presentation: Purified
Product is tested for ELISA (detection), Western blot / Immunoblot.

Properties for PRAME / MAPE

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Melanoma antigen preferentially expressed in tumors, OIP-4, OIP4, OPA-interacting protein 4, Preferentially expressed antigen of melanoma
Presentation Purified
Reactivity Hu
Applications E(detection), WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P78395
Immunogen:
Highly pure (>95%) recombinant human PAME /MAPE C-terminal end (Met321-Asn509) derived from E. coli
GeneID:
23532
Property C-term
Application Western blot: 1-5 μg/ml.
ELISA: 0.1-1 µg/ml.
Background PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumors. The physiological functions of PRAME in normal and tumor cells are unknown, although a role in the regulation of retinoic acid signaling has been proposed. Sequence homology and structural predictions suggest that PRAME is related to the Leucine-rich repeat (LRR) family of proteins, which have diverse functions. PRAME, or „preferentially expressed antigen in melanoma”, was originally identified as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumor-specific cytotoxic T lymphocytes derived from a patient with melanoma. PRAME is synonymous with MAPE (melanoma antigen preferentially expressed in tumors) and OIP4 (OPA-interacting protein 4), and its expression profile defines it as a cancer-testis antigen. Cancer-testis antigens (CTAs) are encoded by non-mutated genes expressed at high levels in germinal tissues and tumors, but which are absent from or detected at low levels in other tissues. PRAME may be somewhat different to other cancer-testis antigens in that it shows some expression in normal tissues such as ovary, adrenal, placenta and endometrium. The C-terminus of human PRAME (amino acids 453-509) was also identified to bind Neisseria gonorrhoeae opacity factors, in this case the OPA-P protein. Thus PRAME is also known as OIP4 (OPA interacting protein).
General Readings
  1. Ikeda H, Lethé B, Lehmann F, van Baren N, Baurain JF, de Smet C, et al. Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor. Immunity. 1997 Feb;6(2):199-208. PubMed PMID: 9047241.
  2. Haqq C, Nosrati M, Sudilovsky D, Crothers J, Khodabakhsh D, Pulliam BL, et al. The gene expression signatures of melanoma progression. Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):6092-7. Epub 2005 Apr 15. PubMed PMID: 15833814. (Free PMC Article available, 4 images available)
  3. Williams JM, Chen GC, Zhu L, Rest RF. Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth. Mol Microbiol. 1998 Jan;27(1):171-86. PubMed PMID: 9466265.
  4. Nakamura Y, Tanaka F, Nagahara H, Ieta K, Haraguchi N, Mimori K, et al. Opa interacting protein 5 (OIP5) is a novel cancer-testis specific gene in gastric cancer. Ann Surg Oncol. 2007 Feb;14(2):885-92. Epub 2006 Dec 7. PubMed PMID: 17151793.
Storage Store lyophilized at 2-8°C for 6 month or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A chromatography
Buffer System:
PBS
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects PRAME / MAPE at C-term.

Accessory Products

Proteins and/or Positive Controls

Proteins for PRAME / MAPE (4 products)

Catalog No. Species Pres. Purity   Source  

PRAME / MAPE (transcript variant 4)

PRAME / MAPE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

PRAME / MAPE (N-term HIS tag, transcript variant 3)

PRAME / MAPE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / $205.00
  OriGene Technologies, Inc.

PRAME / MAPE (full length, N-term HIS tag, transcript variant 5)

PRAME / MAPE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / $205.00
  OriGene Technologies, Inc.

PRAME / MAPE

Sandwich ELISA using the recombinant human PRAME fragment Cat.-No. AR60005PU-N as standard. Human Purified > 98 % by SDS-PAGE and Silver staining E. coli
20 µg / $200.00
  OriGene Technologies GmbH
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