BM6011P Neurofilament M (160 kD) antibody

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0.1 mg / $370.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Mouse anti Hamster, Human, Monkey, Rat, Xenopus Neurofilament M (160 kD) RNF403

BM6011P

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  • BM6011P

Product Description for Neurofilament M (160 kD)

Mouse anti Hamster, Human, Monkey, Rat, Xenopus Neurofilament M (160 kD) RNF403.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Neurofilament M (160 kD)

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide
Presentation Purified
Reactivity Hst, Hu, Mky, Rt, Xen
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone RNF403
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07197
Immunogen:
Neurofilament preparation of calf brain tissue.
GeneID:
4741
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunoblotting.
Immunocytochemistry/Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin-Embedded Tissues.
Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and for 1/100-1/500 for Immunoblotting.
Background Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Concentration 1.0 mg/ml
General Readings
  1. Bauwens LJ, De Groot JC, Ramaekers FC, Veldman JE, Huizing EH. Expression of intermediate filament proteins in the adult human vestibular labyrinth. Ann Otol Rhinol Laryngol. 1992 Jun;101(6):479-86. PubMed PMID: 1376975.
Storage Store undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Hamster, Monkey, Rat and Xenopus
Specificity
Specificity:
RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.
Gene ID 4741

Accessory Products

Proteins and/or Positive Controls

Proteins for Neurofilament M (160 kD) (1 products)

Catalog No. Species Pres. Purity   Source  

Neurofilament M (160 kD) (transcript variant 1)

Neurofilament  M (160 kD) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $788.00
  OriGene Technologies, Inc.
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