DDX0284-HRPO-100 MMP-12 antibody

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0.1 mg / $490.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human MMP-12 701E4.03

Product Description for MMP-12

Mouse anti Human MMP-12 701E4.03.
Presentation: HRP
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for MMP-12

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms HME, ME, MMP12, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase-12
Presentation HRP
Reactivity Hu
Applications E, WB
Clonality Monoclonal
Clone 701E4.03
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Swiss Prot Num:
Recombinant HME.
Application DDX0284P-50 DDX0284P-100 Purified: Capture, Immunoprecipitation, Western Blot. 

Usage recommendation:

*This monoclonal antibody may be used:
Capture: 3μg/ml in Carbonate buffer (pH 9,6).
Detection: 5μg/ml in PBS-BSA-tween.
Positive standard: 1/200 = 10 ng/ml.
*Optimal dilution should be determined by each laboratory for each application.
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is reported only in a few cells, including tissue macrophages and hypertrophic chondrocytes. MMP-12 is critical for invasion and destruction in pathologies such as aneurysm and emphysema. The predicted molecular mass of the HME proenzyme is 54 kDa. HME mRNA and protein were detected in human alveolar macrophages. Similar to murine macrophage metalloelastase, HME readily undergoes NH2- and COOH-terminal processing to a mature 22 kDa form. Both recombinant expressed in Escherichia Coli and native HME derived from human alveolar macrophageconditioned media degraded insoluble elastin. HME is a unique human metalloproteinase that displays elastolytic activity and is expressed in alveolar macrophages; MMP-12 mediates smoke-induced inflammation by releasing TNFα from macrophages, with subsequent endothelial activation, neutrophil influx, and proteolytic matrix breakdown caused by neutrophil-derived proteases. (Demedts IK et al, 2006; Thorax, 61:196-201).
Product Citations
Originator or purchased from resellers:
  1. Demedts IK, Morel-Montero A, Lebecque S, Pacheco Y, Cataldo D, Joos GF, et al. Elevated MMP-12 protein levels in induced sputum from patients with COPD. Thorax. 2006 Mar;61(3):196-201. Epub 2005 Nov 24. PubMed PMID: 16308335. (Free PMC Article available, 7 images available)
  2. Souissi IJ, Billiet L, Cuaz-Pérolin C, Slimane MN, Rouis M. Matrix metalloproteinase-12 gene regulation by a PPAR alpha agonist in human monocyte-derived macrophages. Exp Cell Res. 2008 Nov 1;314(18):3405-14. doi: 10.1016/j.yexcr.2008.09.002. Epub 2008 Sep 18. PubMed PMID: 18823978.
  3. Chaudhuri R, McSharry C, Brady J, Donnelly I, Grierson C, McGuinness S, et al. Sputum matrix metalloproteinase-12 in patients with chronic obstructive pulmonary disease and asthma: relationship to disease severity. J Allergy Clin Immunol. 2012 Mar;129(3):655-663.e8. doi: 10.1016/j.jaci.2011.12.996. Epub 2012 Feb 2. PubMed PMID: 22305682.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
QMA Hyper D ion exchange chromatography
Buffer System:
Tris-NaCl pH 8
Human HME-MMP12. 
*For DDX0284 recognition of the 54kDa form, a pre-treatment of the samples with DTT is required (Demedts IK et al, 2006; Thorax, 61:196-201).

Accessory Products

Proteins and/or Positive Controls

Positive controls for MMP-12 (1 products)

Catalog No. Species Pres. Purity   Source  

MMP12 overexpression lysate

MMP12 overexpression lysate
  OriGene Technologies, Inc.
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