AP33054HR-N LALBA antibody

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1 ml / $530.00
Delivery Time: 10 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human LALBA

AP33054HR-N

Product Description for LALBA

Rabbit anti Human LALBA.
Presentation: HRP
Product is tested for Paraffin Sections, Western blot / Immunoblot, Dot blot, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Properties for LALBA

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms Alpha-lactalbumin, LALBA, LYZL7, Lactose synthase B protein, Lysozyme-like protein 7
Presentation HRP
Reactivity Hu
Applications Dot, E, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00709
Immunogen:
Highly purified alpha-lactalbumin isolated from Human milk.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
3906
Application ELISA. 
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections. 
Dot blot.
Immunoblotting.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of alpha lactalbumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure alpha lactalbumin in serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions 
Histochemical and Cytochemical Use: 1/100-1/250;
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Background Alpha-lactalbumin is one of the major proteins in the alpha region after immunoelectrophoresis of human milk. It has been implicated as a component of the enzyme lactose synthetase which catalyzes the formation of lactose from uridine diphosphate galactose and glucose.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2). 
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.
HRP
Molar radio:
Enzyme/IgG protein molar ratio (F/P): ~1.7
Purification:
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized purified IgG Hyperimmune fraction
Reconstitution:
Restore by adding 1.0 ml of sterile distilled water
Specificity
Specificity:
The defined antibody specificity is directed to alpha-lactalbumin as tested against human milk. Its presence can also be demonstrated in udder tissues. When tested in immunoelectrophoresis and double radial immunodiffusion against serum no reaction is obtained.
Cross-reactivity: Homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has not been tested for cross-reactivity.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for LALBA (2 products)

Catalog No. Species Pres. Purity   Source  

LALBA

LALBA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

LALBA

LALBA Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
  OriGene Technologies, Inc.
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