TA319272 Interleukin-32 / IL32 A antibody

Rabbit polyclonal IL-32A antibody Peroxidase Conjugated

See related secondary antibodies

Search for all "Interleukin-32 / IL32"

0.1 mg / $515.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human Interleukin-32 / IL32

Product Description for Interleukin-32 / IL32

Rabbit anti Human Interleukin-32 / IL32.
Properties: A
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Interleukin-32 / IL32

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IL-32, NK4, Natural killer cells protein 4, TAIF, Tumor necrosis factor alpha-inducing factor
Presentation HRP
Reactivity Hu
Applications C, E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P24001
Immunogen:
Full length recombinant human IL-32A protein
GeneID:
9235
Property A
Application ELISA: 1/10,000 - 1/50,000.
Western Blot: 1/1,000 - 1/5,000.
Immunohistochemistry: 1/500 - 1/2,500.
Other Dilution: User Optimized
Background IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Nishida,A., Andoh,A., Inatomi,O. and Fujiyama,Y. (2009) Interleukin-32 expression in the pancreas. J. Biol. Chem. 284 (26), 17868-17876.
  2. Kobayashi H, Lin PC. Molecular characterization of IL-32 in human endothelial cells. Cytokine. 2009 Jun;46(3):351-8. doi: 10.1016/j.cyto.2009.03.007. Epub 2009 Apr 11. PubMed PMID: 19364659. (Free PMC Article available, 6 images available)
  3. Li W, Yang F, Liu Y, Gong R, Liu L, Feng Y, et al. Negative feedback regulation of IL-32 production by iNOS activation in response to dsRNA or influenza virus infection. Eur J Immunol. 2009 Apr;39(4):1019-24. doi: 10.1002/eji.200838885. PubMed PMID: 19291698.
  4. Nold-Petry CA, Nold MF, Zepp JA, Kim SH, Voelkel NF, Dinarello CA. IL-32-dependent effects of IL-1beta on endothelial cell functions. Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3883-8. doi: 10.1073/pnas.0813334106. Epub 2009 Feb 19. PubMed PMID: 19228941. (Free PMC Article available, 5 images available)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Peroxidase (Horseradish)
HRP
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative
(Do NOT add Sodium Azide!)
State:
Lyophilized
Reconstitution:
Restore with 0.1 mL of deionized water (or equivalent).
Specificity
Specificity:
This antibody reacts to Interleukin-32A.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-32 / IL32 (4 products)

Catalog No. Species Pres. Purity   Source  

Interleukin-32 / IL32 (transcript variant 4)

Interleukin-32 / IL32 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 (transcript variant 8)

Interleukin-32 / IL32 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 (Isoform 4 / alpha) (His-tag)

Interleukin-32 / IL32 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / $820.00
  OriGene Technologies GmbH

Interleukin-32 / IL32 (Isoform 4 / alpha) (His-tag)

Interleukin-32 / IL32 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / $320.00
  OriGene Technologies GmbH

Positive controls for Interleukin-32 / IL32 (8 products)

Catalog No. Species Pres. Purity   Source  

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

IL32 overexpression lysate

IL32 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.
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