AM33333PU-T Hepatocyte Specific Antigen (Hepatocellular Marker) antibody

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20 µg / $108.00
Delivery Time: 1-3 workingdays*
(*) valid for US customers only

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Mouse anti Human Hepatocyte Specific Antigen HSA133


Product Description for Hepatocyte Specific Antigen

Mouse anti Human Hepatocyte Specific Antigen HSA133.
Properties: (Hepatocellular Marker)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Hepatocyte Specific Antigen

Product Category Primary Antibodies
Quantity 20 µg
Presentation Purified
Reactivity Hu
Applications C, ICC/IF
Clonality Monoclonal
Clone HSA133
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

SK-H1A9-2 human hepatocellular carcinoma cells
Property Hepatocellular Marker
Isotype control AM03110PU-N, SM12P
Application Immunofluorescence: 0.5-1 µg/ml. 
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 µg/ml for 30 minutes.
Immunohistochemistry on Frozen
Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Recommended Positive Control: Normal liver or hepatocellular carcinoma (HCC).
Background Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Concentration 0.2 mg/ml
General Readings
  1. Wennerberg AE, Nalesnik MA, Coleman WB. Hepatocyte paraffin 1: a monoclonal antibody that reacts with hepatocytes and can be used for differential diagnosis of hepatic tumors. Am J Pathol. 1993 Oct;143(4):1050-4. PubMed PMID: 7692729. (Free PMC Article available)
  2. Ramos-Vara, J.A., et al. Histochem 2002; J. 34: 397-401.
  3. Fan Z, van de Rijn M, Montgomery K, Rouse RV. Hep par 1 antibody stain for the differential diagnosis of hepatocellular carcinoma: 676 tumors tested using tissue microarrays and conventional tissue sections. Mod Pathol. 2003 Feb;16(2):137-44. PubMed PMID: 12591966.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
Monoclonal Antibody HSA133 stains Human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellul ar carcinoma biopsies and cell lines are found to bind this MAb on the cell surface.
HSA133 str ongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraf ormaldehyde fixed cell preparations.
Cellular LocalizationCell Surface.

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