AP01311PU-N EDG5 / S1P2 antibody

See related secondary antibodies

Search for all "EDG5 / S1P2"

0.1 mg / $370.00
Delivery Time: 10 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human, Mouse, Rat EDG5 / S1P2

Product Description for EDG5 / S1P2

Rabbit anti Human, Mouse, Rat EDG5 / S1P2.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for EDG5 / S1P2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms AGR16, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13, H218, LPB2, S1P receptor 2, S1PR2, Sphingosine 1-phosphate receptor 2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~39 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O95136
Immunogen:
Synthetic peptide corresponding to amino acids 271-320 of Human EDG-5.
GeneID:
9294
Application Western blot: 1/500-1/1000.
Immunofluorescence: 1/50-1/200.
Background The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Cruz-Orengo L, Daniels BP, Dorsey D, Basak SA, Grajales-Reyes JG, McCandless EE, et al. Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest. 2014 May 8. pii: 73408. doi: 10.1172/JCI73408. PubMed PMID: 24812668.
Originator or purchased from resellers:
  1. Huang K, Liu W, Lan T, Xie X, Peng J, Huang J, et al. Berberine reduces fibronectin expression by suppressing the S1P-S1P2 receptor pathway in experimental diabetic nephropathy models. PLoS One. 2012;7(8):e43874. doi: 10.1371/journal.pone.0043874. Epub 2012 Aug 24. PubMed PMID: 22937115. (Free PMC Article available, 8 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific immunogen
Buffer System:
Phosphate buffered saline (PBS), pH~7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Rat, Mouse.
Specificity
Specificity:
This antibody detects endogenous levels of EDG-5 protein (region surrounding Leu300).
Gene ID 9294
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for EDG5 / S1P2 (1 products)

Catalog No. Species Pres. Purity   Source  

EDG5 / S1P2

EDG5 / S1P2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.
  • LinkedIn