SM1424P Cyclin D1 antibody

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0.1 mg / $480.00
Delivery Time: 3-5 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human Cyclin D1 CD1.1

SM1424P

Product Description for Cyclin D1

Mouse anti Human Cyclin D1 CD1.1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Cyclin D1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1
Presentation Purified
Reactivity Hu
Applications C, E, F, IP, P, WB
Clonality Monoclonal
Clone CD1.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P24385
Immunogen:
Human Cyclin D1. Spleen cells from immunised mice were fused with cells of the mouse Sp-2 myeloma cell line.
GeneID:
595
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry (neat): Membrane permeabilisation is required for this application.
ELISA.
Immunoprecipitation.
Western Blot: 1/500.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: 1/1000-1/10000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose.
Recommended Positive Control: Small intestine, colon.
Background Cyclin D1 a 36kD nuclear protein. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy, and over expression has been linked to poor prognosis.
During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2.
In a recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Cyclin D1 is also involved in development and cancer.
Concentration 1.0 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A.
Buffer System:
PBS containing 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
This antibody recognises the Cyclin D1.
Species:
Human and Rat.
Gene ID 595
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Accessory Products

Proteins and/or Positive Controls

Proteins for Cyclin D1 (2 products)

Catalog No. Species Pres. Purity   Source  

Cyclin D1

Cyclin D1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / $680.00

Special Price: 20 µg / $398.00

  OriGene Technologies, Inc.

Cyclin D1 (full length, C-term flag tag)

Cyclin D1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / $399.00
  OriGene Technologies, Inc.
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