DP137-05 Cyclin B1 antibody

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0.5 ml / $360.00
Delivery Time: 2-3 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human, Mouse, Rat Cyclin B1

Product Description for Cyclin B1

Rabbit anti Human, Mouse, Rat Cyclin B1.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for Cyclin B1

Product Category Primary Antibodies
Target Category
Quantity 0.5 ml
Synonyms CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1
Presentation Purified
Reactivity Hu, Ms, Rt
Applications P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14635
Immunogen:
A synthetic peptide derived from C-terminus of human cyclin B1 protein
GeneID:
891
Application Immunohistochemistry on formalin-fixed paraffin embedded sections:
dilute 1:50-1:100 in an ABC method (incubation time: 30 minutes at room temperature).
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Recommended positive control: Breast carcinoma.
Background In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid purified Ig fraction containing sodium azide as preservative
Purified
Specificity
Specificity:
Reacts to Cyclin B1.
Cellular localization: cytoplasmic.
Species:
Human, Mouse, Rat.
Gene ID 891
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Accessory Products

Proteins and/or Positive Controls

Proteins for Cyclin B1 (2 products)

Catalog No. Species Pres. Purity   Source  

Cyclin B1 (1-433, His-tag)

Cyclin B1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / $790.00
  Acris Antibodies GmbH

Cyclin B1 (1-433, His-tag)

Cyclin B1 Human Purified > 80 % by SDS - PAGE E. coli
20 µg / $340.00
  Acris Antibodies GmbH

Positive controls for Cyclin B1 (1 products)

Catalog No. Species Pres. Purity   Source  

CCNB1 overexpression lysate

CCNB1 overexpression lysate
0.1 mg / $280.00
  OriGene Technologies, Inc.
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