AP09487PU-N Cyclin B1 pSer147 antibody

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0.1 ml / $310.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

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Rabbit anti Human Cyclin B1

AP09487PU-N

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Product Description for Cyclin B1

Rabbit anti Human Cyclin B1.
Properties: pSer147
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for Cyclin B1

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1
Presentation Aff - Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14635
Immunogen:
Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-Sp-D-V).
GeneID:
891
Property pSer147
Application Immunofluorescence: 1/100 - 1/200.
Immunohistochemistry on Paraffin Sections: 1/50 - 1/100.
Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Background In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Concentration 1.0 mg/ml
General Readings
  1. Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
  2. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
  3. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Serine 147.
Species:
Human.
Gene ID 891
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Accessory Products

Proteins and/or Positive Controls

Proteins for Cyclin B1 (2 products)

Catalog No. Species Pres. Purity   Source  

Cyclin B1 (1-433, His-tag)

Cyclin B1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / $790.00
  Acris Antibodies GmbH

Cyclin B1 (1-433, His-tag)

Cyclin B1 Human Purified > 80 % by SDS - PAGE E. coli
20 µg / $340.00
  Acris Antibodies GmbH

Positive controls for Cyclin B1 (1 products)

Catalog No. Species Pres. Purity   Source  

CCNB1 overexpression lysate

CCNB1 overexpression lysate
0.1 mg / $280.00
  OriGene Technologies, Inc.
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