AP01169PU-N Cyclin B1 antibody

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0.1 mg / $330.00
Delivery Time: 10 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human, Mouse, Rat Cyclin B1

Product Description for Cyclin B1

Rabbit anti Human, Mouse, Rat Cyclin B1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Cyclin B1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 60 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14635
Immunogen:
Synthetic peptide, corresponding to amino acids 110-160 of Human Cyclin B1.
GeneID:
891
Application Western Blot: 1/500 - 1/1000.
Background In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase.
This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex .
Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
Concentration 1.0 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch
Format
Purification:
Affinity Chromatography using epitope-specific immunogen.
Buffer System:
Phosphate buffered saline (PBS), pH~7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of Cyclin B1 protein.(region surrounding Gln144)
Gene ID 891
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Accessory Products

Proteins and/or Positive Controls

Proteins for Cyclin B1 (2 products)

Catalog No. Species Pres. Purity   Source  

Cyclin B1 (1-433, His-tag)

Cyclin B1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / $790.00
  Acris Antibodies GmbH

Cyclin B1 (1-433, His-tag)

Cyclin B1 Human Purified > 80 % by SDS - PAGE E. coli
20 µg / $340.00
  Acris Antibodies GmbH

Positive controls for Cyclin B1 (1 products)

Catalog No. Species Pres. Purity   Source  

CCNB1 overexpression lysate

CCNB1 overexpression lysate
0.1 mg / $280.00
  OriGene Technologies, Inc.
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