DDX0321P-100 CD39 / ENTPD1 antibody

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0.1 mg / $440.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human CD39 / ENTPD1 AC2.5

Product Description for CD39 / ENTPD1

Mouse anti Human CD39 / ENTPD1 AC2.5.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for CD39 / ENTPD1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms ATPDase, Ecto-ATP diphosphohydrolase, Ecto-apyrase, Ectonucleoside triphosphate diphosphohydrolase 1, Lymphoid cell activation antigen, NTPDase 1
Presentation Purified
Reactivity Hu
Applications C, IP, WB
Clonality Monoclonal
Clone AC2.5
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P49961
Immunogen:
EBV-transformed human B lymphoblastoid cell line.
GeneID:
953
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application DDX0321P-50 DDX0321P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, Immunoprecipitation, Western Blot.
DDX032A488-50 DDX0321A488-100 AlexaFluor®488: FACS surface, Immunofluorescence.
DDX0321A546-50 DDX0321A546-100 AlexaFluor®546: Immunofluorescence.
DDX0321A647-50 DDX0321A647-100 AlexaFluor®647: Surface and Intracyto Flow cytometry
DDX0321B-50 DDX0321B-100 Biotin:  Western Blot, ImmunoHistoChemistry frozen sections. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background CD39, also known as ENTPD1, is a 58KDa multi-pass membrane protein belonging to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. AC2.5 was generated after mouse immunization with EBV-transformed B cells. (Rowe M et al, Int J Cancer. 1982 Apr 15;29(4):373- 81).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Ingvarsson S, Dahlenborg K, Carlsson R, Borrebaeck CA. Co-ligation of CD44 on naive human tonsillar B cells induces progression towards a germinal center phenotype. Int Immunol. 1999 May;11(5):739-44. PubMed PMID: 10330279.
  2. Dahlenborg K, Pound JD, Gordon J, Borrebaeck CA, Carlsson R. Signals sustaining human immunoglobulin V gene hypermutation in isolated germinal centre B cells. Immunology. 2000 Oct;101(2):210-7. PubMed PMID: 11012774. (Free PMC Article available, 4 images available)
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles. 
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human CD39.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD39 / ENTPD1 (1 products)

Catalog No. Species Pres. Purity   Source  

CD39 / ENTPD1 (transcript variant 1)

CD39 / ENTPD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Positive controls for CD39 / ENTPD1 (1 products)

Catalog No. Species Pres. Purity   Source  

ENTPD1 overexpression lysate

ENTPD1 overexpression lysate
0.1 mg / $315.00
  OriGene Technologies, Inc.
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