SM1097AS CD35 / C3BR antibody

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50 µg / $425.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human, Monkey, Baboon CD35 / C3BR E11

Product Description for CD35 / C3BR

Mouse anti Human, Monkey, Baboon CD35 / C3BR E11.
Presentation: Azide Free
Product is tested for Frozen Sections, Paraffin Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for CD35 / C3BR

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms C3b/C4b receptor, CR1, Complement receptor type 1
Presentation Azide Free
Reactivity Bab, Hu, Mky
Applications C, F, P, WB
Clonality Monoclonal
Clone E11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17927
Immunogen:
Human acute monocytic leukaemia cells.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
GeneID:
1378
Application Flow Cytometry: 1/50 - 1/100.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Western Blot.
Background CD35 is a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.
Concentration 1 mg/ml
General Readings
  1. Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236-43. PubMed PMID: 6368248.
  2. Yoshino N, Ami Y, Terao K, Tashiro F, Honda M. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110. PubMed PMID: 10889948.
  3. Birmingham, D. J. et al. (1996) The baboon erythrocyte complement receptor is glycophosphatidyl/insitollinked protein encoded by homologue of the human CR1 - like genetic element. J. Immunol. 157: 2586 - 2592.
  4. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dörries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997 Dec 1;29(4):351-62. PubMed PMID: 9415418.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH 7.4
Preservatives:
None
Stabilizers:
None
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Azide Free
Specificity
Specificity:
This antibody is a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement.
Species:
Human, Cynomolgus monkey, Baboon, Rhesus Monkey.
Gene ID 1378

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