AM50104PU-S CD35 / C3BR antibody

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0.1 mg / $430.00
Delivery Time: 1-3 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human, Monkey, Baboon CD35 / C3BR SPM554

Product Description for CD35 / C3BR

Mouse anti Human, Monkey, Baboon CD35 / C3BR SPM554.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for CD35 / C3BR

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms C3b/C4b receptor, CR1, Complement receptor type 1
Presentation Purified
Reactivity Bab, Hu, Mky
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone SPM554
Host Mouse
Isotype IgG1
Molecular weight 210-220kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17927
Immunogen:
Intact human monocytes.
GeneID:
1378
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 1-2 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Follicular dendritic cells (FDC) in tonsil.
Concentration 0.2 mg/ml
General Readings
  1. Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236-43. PubMed PMID: 6368248.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35.
This Monoclonal Antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity.
This Monoclonal Antibodyis is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. It labels follicular dendritic cells and follicular dendritic cell sarcoma.
Cellular Localization: Cell surface.
Species:
Human, Baboon, Cynomolgus Monkey, Rhesus Monkey.

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