DDX0476A546-100 CD283 / TLR3 (LRR7-LRR8) antibody

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0.1 mg / $530.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human CD283 / TLR3 1213F10 / TLR3.3

Product Description for CD283 / TLR3

Mouse anti Human CD283 / TLR3 1213F10 / TLR3.3.
Properties: (LRR7-LRR8)
Presentation: Alexa Fluor 546
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for CD283 / TLR3

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Toll-like receptor 3
Presentation Alexa Fluor 546
Reactivity Hu
Applications F, ICC/IF, IP, WB
Clonality Monoclonal
Clone 1213F10 / TLR3.3
Host Mouse
Isotype IgG1
Molecular weight 135KDa, 130KDa, 60KDa
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
O15455
Immunogen:
HEK-293 stably transfected with huTLR3+rhu TLR3 ECD (R&D)
Property LRR7-LRR8
Application FACS intracellular, ImmunoFluorescence, Immunoprecipitation, Western Blot. 

Usage recommendation:

*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background The Toll-like receptor (TLR) family are considered as key for sensing microbial infections and triggering inflammatory and innate responses. 3 novel mAbs were generated against TLR3 that support the emergence of an alternative model for TLR3 biology: TLR3 full-length (TLR3FL, 130KDa) corresponds to the TLR3 present in ER, which in resting cells is steadily converted within the Golgi into fully glycosylated TLR3 (TLR3FL+, 135KDa) and then rapidly cleaved by cathepsins in the endosome/lysosome into 2 long-lived associated fragments (72 KDa and 60KDa). (Toscano F et al, J. Immunol, 2013, 190: 764–773).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Toscano F, Estornes Y, Virard F, Garcia-Cattaneo A, Pierrot A, Vanbervliet B, et al. Cleaved/associated TLR3 represents the primary form of the signaling receptor. J Immunol. 2013 Jan 15;190(2):764-73. doi: 10.4049/jimmunol.1202173. Epub 2012 Dec 19. PubMed PMID: 23255358.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 546
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
Specificity
Specificity:
Human TLR3. 
Do not recognize Mouse TLR3 (WB).

Accessory Products

Proteins and/or Positive Controls

Proteins for CD283 / TLR3 (3 products)

Catalog No. Species Pres. Purity   Source  

CD283 / TLR3

CD283 / TLR3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
25 µg / $230.00
  OriGene Technologies, Inc.

CD283 / TLR3

CD283 / TLR3 Human Purified > 95 % pure by SDS-PAGE and HPLC analyses HEK293 cells
  OriGene Technologies GmbH

CD283 / TLR3

CD283 / TLR3 Human Purified > 95 % pure by SDS-PAGE and HPLC analyses HEK293 cells
  OriGene Technologies GmbH

Positive controls for CD283 / TLR3 (1 products)

Catalog No. Species Pres. Purity   Source  

TLR3 overexpression lysate

TLR3 overexpression lysate
  OriGene Technologies, Inc.
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