DDX0470P-100 CD283 / TLR3 antibody

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0.1 mg / $440.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

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Mouse anti Canine, Human, Mouse, Rat CD283 / TLR3 619F7.06

DDX0470P-100

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Product Description for CD283 / TLR3

Mouse anti Canine, Human, Mouse, Rat CD283 / TLR3 619F7.06.
Presentation: Purified
Product is tested for Paraffin Sections, Flow Cytometry, Frozen Sections, Western blot / Immunoblot.

Properties for CD283 / TLR3

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Toll-like receptor 3
Presentation Purified
Reactivity Can, Hu, Ms, Rt
Applications C, F, P, WB
Clonality Monoclonal
Clone 619F7.06
Host Mouse
Isotype IgM
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
O15455
Immunogen:
Human recombinant TLR3 in eukaryotic cells (pUNO-hTLR3)
Isotype control SM13P
Application DDX0470P-50 / DDX0470P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Western Blot.
DDX0470A488-50 / DDX0470A488-100
Alexa-fluor®488: FACS intracellular, ImmunoFluorescence. 
DDX0470A546-50 / DDX0470A546-100
Alexa-fluor®546: ImmunoFluorescence.
DDX0470A647-50 / DDX0470A647-100
Alexa- fluor®647: FACS intracellular. 
DDX0470B-50 / DDX0470B-100 Biotin: Western Blot.


Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background The Toll-like receptor (TLR) family has recently emerged as key for sensing microbial infections and triggering inflammatory and innate responses. TLR share structural and functional homologies, as they are all able to detect micro-organisms through direct recognition of conserved Pathogen Associated Molecular Patterns (PAMPs) such as bacterial LPS or viral dsRNA. Ligand recognition is mediated through the Cterminal Leucine Rich Repeat region, while signalling involves the N-terminal Toll/Interleukin-1 Receptor (TIR) domain. TLR3 has been identified as a receptor for dsRNA. TLR3 is the only TLR that signals exclusively through the TIR-containing adaptor TRIF (TIR-containing adaptor inducing IFNβ), which activates the transcription factors IRF3 and NFκB, and triggers type I interferon secretion. TLR3 is expressed in various cell types, such as fibroblasts, dendritic cells (DCs), or NK cells. While TLR3 is expressed at the cell surface in fibroblasts, it is mostly found in intracytoplasmic vesicles in DCs. (Akira S et al, 2004, Nat Rev Immunol, 4: 499-511)
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T. TLR3 can directly trigger apoptosis in human cancer cells. J Immunol. 2006 Apr 15;176(8):4894-901. PubMed PMID: 16585585.
  2. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
  3. Bonnefont-Rebeix C, Marchal T, Bernaud J, Pin JJ, Leroux C, Lebecque S, et al. Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC). Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. Epub 2007 Apr 24. PubMed PMID: 17521746.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human TLR3 (intracytoplasmic).
Species cross- reactivity: Rat, Dog, Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD283 / TLR3 (3 products)

Catalog No. Species Pres. Purity   Source  

CD283 / TLR3

CD283 / TLR3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
25 µg / $230.00
  OriGene Technologies, Inc.

CD283 / TLR3

CD283 / TLR3 Human Purified > 95 % pure by SDS-PAGE and HPLC analyses HEK293 cells
  OriGene Technologies GmbH

CD283 / TLR3

CD283 / TLR3 Human Purified > 95 % pure by SDS-PAGE and HPLC analyses HEK293 cells
  OriGene Technologies GmbH

Positive controls for CD283 / TLR3 (1 products)

Catalog No. Species Pres. Purity   Source  

TLR3 overexpression lysate

TLR3 overexpression lysate
  OriGene Technologies, Inc.
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