DDX0363B-100 CD207 / Langerin antibody

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0.1 mg / $340.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

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Mouse anti Canine, Human CD207 / Langerin DCGM4/122D5.03


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  • DDX0363B-100

Product Description for CD207 / Langerin

Mouse anti Canine, Human CD207 / Langerin DCGM4/122D5.03.
Presentation: Biotin
Product is tested for Flow Cytometry, Frozen Sections, Western blot / Immunoblot.

Properties for CD207 / Langerin

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms C-type lectin domain family 4 member K, CLEC4K, Dendritic Cell Marker
Presentation Biotin
Reactivity Can, Hu
Applications C, F, WB
Clonality Monoclonal
Clone DCGM4/122D5.03
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Swiss Prot Num:
In vitro-derived human DCs from CD34+ cells in GM-CSF+TNFα
Isotype control SM10B (for use in human samples)
Application DDX0363P-50 DDX0363P-100 Purified: Internalization, Flow cytometry, IHC.
DDX0363A488-50 DDX0363A488-100 Alexa-fluor®488: Flow cytometry, IF.
DDX0363A546-50 DDX0363A546-100 Alexa-fluor®546: IF.
DDX0363A647-50 DDX0363A647-100 Alexa- fluor®647: Flow cytometry.
DDX0363B-50 DDX0363B-100 Biotin: Western blot. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined y each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 ; Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Valladeau J, Duvert-Frances V, Pin JJ, Dezutter-Dambuyant C, Vincent C, Massacrier C, et al. The monoclonal antibody DCGM4 recognizes Langerin, a protein specific of Langerhans cells, and is rapidly internalized from the cell surface. Eur J Immunol. 1999 Sep;29(9):2695-704. PubMed PMID: 10508244.
  2. Ito T, Inaba M, Inaba K, Toki J, Sogo S, Iguchi T, et al. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells. J Immunol. 1999 Aug 1;163(3):1409-19. PubMed PMID: 10415041.
  3. Valladeau J, Ravel O, Dezutter-Dambuyant C, Moore K, Kleijmeer M, Liu Y, et al. Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity. 2000 Jan;12(1):71-81. PubMed PMID: 10661407.
  4. Bobr A, Olvera-Gomez I, Igyarto BZ, Haley KM, Hogquist KA, Kaplan DH. Acute ablation of Langerhans cells enhances skin immune responses. J Immunol. 2010 Oct 15;185(8):4724-8. doi: 10.4049/jimmunol.1001802. Epub 2010 Sep 20. PubMed PMID: 20855870. (Free PMC Article available, 4 images available)
  5. de Jong MA, de Witte L, Taylor ME, Geijtenbeek TB. Herpes simplex virus type 2 enhances HIV-1 susceptibility by affecting Langerhans cell function. J Immunol. 2010 Aug 1;185(3):1633-41. doi: 10.4049/jimmunol.0904137. Epub 2010 Jun 30. PubMed PMID: 20592277.
  6. Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013 Jan 1;190(1):27-35. doi: 10.4049/jimmunol.1201082. Epub 2012 Dec 3. PubMed PMID: 23209325.
  7. Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development. 2012 Nov;139(22):4210-9. doi: 10.1242/dev.083477. Epub 2012 Oct 3. PubMed PMID: 23034637. (Free PMC Article available, 4 images available)
  8. Hitzler M, Majdic O, Heine G, Worm M, Ebert G, Luch A, et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One. 2012;7(10):e46776. doi: 10.1371/journal.pone.0046776. Epub 2012 Oct 9. PubMed PMID: 23056446. (Free PMC Article available, 6 images available)
  9. Debeer S, Le Luduec JB, Kaiserlian D, Laurent P, Nicolas JF, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol. 2013 Jul-Aug;23(4):456-66. doi: 10.1684/ejd.2013.2060. PubMed PMID: 24047577.
  10. Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9;209(4):653-60. doi: 10.1084/jem.20111457. Epub 2012 Mar 19. PubMed PMID: 22430490. (Free PMC Article available, 7 images available)
  11. Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, et al. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J Exp Med. 2009 Jan 16;206(1):169-81. doi: 10.1084/jem.20081747. Epub 2009 Jan 12. PubMed PMID: 19139172. (Free PMC Article available, 8 images available)
  12. Huch JH, Cunningham AL, Arvin AM, Nasr N, Santegoets SJ, Slobedman E, et al. Impact of varicella-zoster virus on dendritic cell subsets in human skin during natural infection. J Virol. 2010 Apr;84(8):4060-72. doi: 10.1128/JVI.01450-09. Epub 2010 Feb 3. PubMed PMID: 20130046. (Free PMC Article available, 8 images available)
  13. van de Laar L, van den Bosch A, van der Kooij SW, Janssen HL, Coffer PJ, van Kooten C, et al. A nonredundant role for canonical NF-κB in human myeloid dendritic cell development and function. J Immunol. 2010 Dec 15;185(12):7252-61. doi: 10.4049/jimmunol.1000672. Epub 2010 Nov 12. PubMed PMID: 21076069.
  14. Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-β1-dependent human epidermal Langerhans cells. J Invest Dermatol. 2011 Oct;131(10):2049-57. doi: 10.1038/jid.2011.164. Epub 2011 Jun 16. PubMed PMID: 21677668.
  15. Bigley V, Haniffa M, Doulatov S, Wang XN, Dickinson R, McGovern N, et al. The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency. J Exp Med. 2011 Feb 14;208(2):227-34. doi: 10.1084/jem.20101459. Epub 2011 Jan 17. PubMed PMID: 21242295. (Free PMC Article available, 5 images available)
  16. Fujita H, Shemer A, Suárez-Fariñas M, Johnson-Huang LM, Tintle S, Cardinale I, et al. Lesional dendritic cells in patients with chronic atopic dermatitis and psoriasis exhibit parallel ability to activate T-cell subsets. J Allergy Clin Immunol. 2011 Sep;128(3):574-82.e1-12. doi: 10.1016/j.jaci.2011.05.016. Epub 2011 Jun 25. PubMed PMID: 21704361.
  17. van der Vlist M, de Witte L, de Vries RD, Litjens M, de Jong MA, Fluitsma D, et al. Human Langerhans cells capture measles virus through Langerin and present viral antigens to CD4⁺ T cells but are incapable of cross-presentation. Eur J Immunol. 2011 Sep;41(9):2619-31. doi: 10.1002/eji.201041305. Epub 2011 Aug 8. PubMed PMID: 21739428.
  18. Ouwehand K, Spiekstra SW, Waaijman T, Scheper RJ, de Gruijl TD, Gibbs S. Technical advance: Langerhans cells derived from a human cell line in a full-thickness skin equivalent undergo allergen-induced maturation and migration. J Leukoc Biol. 2011 Nov;90(5):1027-33. doi: 10.1189/jlb.0610374. Epub 2011 Jun 22. PubMed PMID: 21697260.
  19. Stary G, Klein I, Bauer W, Koszik F, Reininger B, Kohlhofer S, et al. Glucocorticosteroids modify Langerhans cells to produce TGF-β and expand regulatory T cells. J Immunol. 2011 Jan 1;186(1):103-12. doi: 10.4049/jimmunol.1002485. Epub 2010 Dec 6. PubMed PMID: 21135170.
  20. Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, et al. HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies. J Virol. 2011 Jan;85(2):1077-85. doi: 10.1128/JVI.01619-10. Epub 2010 Nov 17. PubMed PMID: 21084491. (Free PMC Article available, 4 images available)
  21. Schwingshackl P, Obermoser G, Nguyen VA, Fritsch P, Sepp N, Romani N. Distribution and maturation of skin dendritic cell subsets in two forms of cutaneous T-cell lymphoma: mycosis fungoides and Sézary syndrome. Acta Derm Venereol. 2012 May;92(3):269-75. doi: 10.2340/00015555-1220. PubMed PMID: 22678564.
  22. Haniffa M, Shin A, Bigley V, McGovern N, Teo P, See P, et al. Human tissues contain CD141hi cross-presenting dendritic cells with functional homology to mouse CD103+ nonlymphoid dendritic cells. Immunity. 2012 Jul 27;37(1):60-73. doi: 10.1016/j.immuni.2012.04.012. Epub 2012 Jul 12. PubMed PMID: 22795876. (Free PMC Article available, 9 images available)
  23. Mayumi N, Watanabe E, Norose Y, Watari E, Kawana S, Geijtenbeek TB, et al. E-cadherin interactions are required for Langerhans cell differentiation. Eur J Immunol. 2013 Jan;43(1):270-80. doi: 10.1002/eji.201242654. Epub 2012 Dec 11. PubMed PMID: 23135957. (Free PMC Article available, 7 images available)
  24. Schuster C, Mildner M, Mairhofer M, Bauer W, Fiala C, Prior M, et al. Human embryonic epidermis contains a diverse Langerhans cell precursor pool. Development. 2014 Feb;141(4):807-15. doi: 10.1242/dev.102699. PubMed PMID: 24496618.
  25. Bonetti MI, Bacci S, Santosuosso M, Mazzanti B, Aldinucci A, Ballerini C, et al. Rosiglitazone promotes the differentiation of Langerhans cells and inhibits that of other dendritic cell types from CD133 positive hematopoietic precursors. Histol Histopathol. 2014 Mar;29(3):323-32. Epub 2013 Jul 24. PubMed PMID: 23881602.
  26. Lenormand C, Spiegelhalter C, Cinquin B, Bardin S, Bausinger H, Angénieux C, et al. Birbeck granule-like "organized smooth endoplasmic reticulum" resulting from the expression of a cytoplasmic YFP-tagged langerin. PLoS One. 2013;8(4):e60813. doi: 10.1371/journal.pone.0060813. Epub 2013 Apr 5. PubMed PMID: 23577166. (Free PMC Article available, 7 images available)
  27. Welty NE, Staley C, Ghilardi N, Sadowsky MJ, Igyártó BZ, Kaplan DH. Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism. J Exp Med. 2013 Sep 23;210(10):2011-24. doi: 10.1084/jem.20130728. Epub 2013 Sep 9. PubMed PMID: 24019552. (Free PMC Article available, 7 images available)
  28. Lommatzsch M, Kraeft U, Troebs L, Garbe K, Bier A, Stoll P, et al. Fluticasone impact on airway dendritic cells in smokers: a randomized controlled trial. Respir Res. 2013 Oct 29;14:114. doi: 10.1186/1465-9921-14-114. PubMed PMID: 24168756. (Free PMC Article available, 5 images available)
  29. Lommatzsch M et al, 2013, supp S1
  30. Flacher V, Tripp CH, Mairhofer DG, Steinman RM, Stoitzner P, Idoyaga J, et al. Murine Langerin+ dermal dendritic cells prime CD8+ T cells while Langerhans cells induce cross-tolerance. EMBO Mol Med. 2014 Aug 1;6(9):1191-204. doi: 10.15252/emmm.201303283. PubMed PMID: 25085878. (Free PMC Article available)
  31. Mayer WJ, Irschick UM, Moser P, Wurm M, Huemer HP, Romani N, et al. Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4459-67. PubMed PMID: 17898266.
  32. Stoll P, Heinz AS, Bratke K, Bier A, Garbe K, Kuepper M, et al. Impact of smoking on dendritic cell phenotypes in the airway lumen of patients with COPD. Respir Res. 2014 Apr 18;15:48. doi: 10.1186/1465-9921-15-48. PubMed PMID: 24742278. (Free PMC Article available, 4 images available)
  33. Stoll P et al, file S1, 2014
  34. Larbi A, Mitjavila-Garcia MT, Flamant S, Valogne Y, Clay D, Usunier B, et al. Generation of multipotent early lymphoid progenitors from human embryonic stem cells. Stem Cells Dev. 2014 Dec 15;23(24):2983-95. doi: 10.1089/scd.2014.0171. PubMed PMID: 24955741.
  35. Nasr N, Lai J, Botting RA, Mercier SK, Harman AN, Kim M, et al. Inhibition of two temporal phases of HIV-1 transfer from primary Langerhans cells to T cells: the role of langerin. J Immunol. 2014 Sep 1;193(5):2554-64. doi: 10.4049/jimmunol.1400630. Epub 2014 Jul 28. PubMed PMID: 25070850.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
Human Langerin (extracellular epitope in Carbohydrate Recognition domain). 
Species cross-reactivity: Dog.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD207 / Langerin (5 products)

Catalog No. Species Pres. Purity   Source  

CD207 / Langerin

CD207 / Langerin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

CD207 / Langerin (65-328, His-tag)

CD207 / Langerin Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / $820.00
  OriGene Technologies GmbH

CD207 / Langerin (65-328, His-tag)

CD207 / Langerin Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / $320.00
  OriGene Technologies GmbH

CD207 / Langerin (65-328, His-tag)

CD207 / Langerin Human Purified > 90 % by SDS - PAGE
0.25 mg / $1,070.00
  OriGene Technologies GmbH

CD207 / Langerin (65-328, His-tag)

CD207 / Langerin Human Purified > 90 % by SDS - PAGE
50 µg / $320.00
  OriGene Technologies GmbH

Positive controls for CD207 / Langerin (1 products)

Catalog No. Species Pres. Purity   Source  

CD207 overexpression lysate

CD207 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.
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