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AM50206PU-T CD11b antibody

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20 µg / $240.00
Delivery Time: 1-3 working days*
(*) valid for US customers only

View Datasheet

Quick Overview

Rat anti Chimpanzee, Human, Mouse, Rabbit, Baboon CD11b M1/70

AM50206PU-T

Product Description for CD11b

Rat anti Chimpanzee, Human, Mouse, Rabbit, Baboon CD11b M1/70.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for CD11b

Product Category Primary Antibodies
Target Category
Quantity 20 µg
Synonyms CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Presentation Purified
Reactivity Bab, Chimp, Hu, Ms, Rb
Applications C, ICC/IF, IP
Clonality Monoclonal
Clone M1/70
Host Rat
Isotype IgG2b
Molecular weight 95kDa & 170kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11215
Immunogen:
B10 Mouse spleen cells enriched for T lymphocytes.
GeneID:
3684
Application ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 0.5-1 μg/ml.
Functional Studies: Use Antibody without BSA and Azide.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
Positive Control: Monocytes & granulocytes, Lymph nodes and tonsils.
Concentration 0.2 mg/ml
General Readings
  1. Springer T, Galfrè G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539-51. PubMed PMID: 81133.
  2. Ault KA, Springer TA. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981 Jan;126(1):359-64. PubMed PMID: 7451976.
  3. Springer TA, Davignon D, Ho MK, Kürzinger K, Martz E, Sanchez-Madrid F. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunol Rev. 1982;68:171-95. PubMed PMID: 6184305.
  4. Ho MK, Springer TA. Biosynthesis and assembly of the alpha and beta subunits of Mac-1, a macrophage glycoprotein associated with complement receptor function. J Biol Chem. 1983 Mar 10;258(5):2766-9. PubMed PMID: 6338004.
  5. Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983 Apr;111(1):112-24. PubMed PMID: 6340516. (Free PMC Article available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ∫2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/∫2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Cellular Localization: Cell surface.
Species:
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit and Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD11b (4 products)

Catalog No. Species Pres. Purity   Source  

CD11b (transcript variant 2)
CD11b Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / $788.00
  OriGene Technologies, Inc.

CD11b
CD11b Human Purified E. coli
  OriGene Technologies GmbH

CD11b
CD11b Human Purified E. coli
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CD11b
CD11b Human Purified E. coli
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Positive controls for CD11b (1 products)

Catalog No. Species Pres. Purity   Source  

ITGAM overexpression lysate
ITGAM overexpression lysate
0.1 mg / $495.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for CD11b
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