AM50206PU-T CD11b antibody

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20 µg / $240.00
Delivery Time: 1-3 working days*
(*) valid for US customers only

Quick Overview

Rat anti Chimpanzee, Human, Mouse, Rabbit, Baboon CD11b M1/70

AM50206PU-T

Product Description for CD11b

Rat anti Chimpanzee, Human, Mouse, Rabbit, Baboon CD11b M1/70.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for CD11b

Product Category Primary Antibodies
Target Category
Quantity 20 µg
Synonyms CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Presentation Purified
Reactivity Bab, Chimp, Hu, Ms, Rb
Applications C, ICC/IF, IP
Clonality Monoclonal
Clone M1/70
Host Rat
Isotype IgG2b
Molecular weight 95kDa & 170kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11215
Immunogen:
B10 Mouse spleen cells enriched for T lymphocytes.
GeneID:
3684
Application ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 0.5-1 μg/ml.
Functional Studies: Use Antibody without BSA and Azide.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
Positive Control: Monocytes & granulocytes, Lymph nodes and tonsils.
Concentration 0.2 mg/ml
General Readings
  1. Springer T, Galfrè G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539-51. PubMed PMID: 81133.
  2. Ault KA, Springer TA. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981 Jan;126(1):359-64. PubMed PMID: 7451976.
  3. Springer TA, Davignon D, Ho MK, Kürzinger K, Martz E, Sanchez-Madrid F. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunol Rev. 1982;68:171-95. PubMed PMID: 6184305.
  4. Ho MK, Springer TA. Biosynthesis and assembly of the alpha and beta subunits of Mac-1, a macrophage glycoprotein associated with complement receptor function. J Biol Chem. 1983 Mar 10;258(5):2766-9. PubMed PMID: 6338004.
  5. Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983 Apr;111(1):112-24. PubMed PMID: 6340516. (Free PMC Article available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ∫2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/∫2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Cellular Localization: Cell surface.
Species:
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit and Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD11b (4 products)

Catalog No. Species Pres. Purity   Source  

CD11b (transcript variant 2)

CD11b Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $788.00
  OriGene Technologies, Inc.

CD11b

CD11b Human Purified E. coli
  OriGene Technologies GmbH

CD11b

CD11b Human Purified E. coli
  OriGene Technologies GmbH

CD11b

CD11b Human Purified E. coli
  OriGene Technologies GmbH

Positive controls for CD11b (1 products)

Catalog No. Species Pres. Purity   Source  

ITGAM overexpression lysate

ITGAM overexpression lysate
0.1 mg / $495.00
  OriGene Technologies, Inc.
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