SM2017P CCT8 / TCP1 theta antibody

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0.1 mg / $510.00
Delivery Time: 3-5 working days*
(*) valid for US customers only

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Rat anti Bovine, Human, Mouse, Rabbit CCT8 / TCP1 theta PK/13/72/8k

Product Description for CCT8 / TCP1 theta

Rat anti Bovine, Human, Mouse, Rabbit CCT8 / TCP1 theta PK/13/72/8k.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for CCT8 / TCP1 theta

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CCT theta, CCTQ, KIAA0002, NY-REN-15, Renal carcinoma antigen NY-REN-15, T-complex protein 1 subunit theta
Presentation Aff - Purified
Reactivity Bov, Hu, Ms, Rb
Applications IP, WB
Clonality Monoclonal
Clone PK/13/72/8k
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P50990
GeneID:
10694
Application Immunoprecipitation.
Western Blot: 1/250-1/500. This antibody detects a band of approximately 60kD in lysates of heat shocked Hela cells.
Background The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Concentration 1.0 mg/ml
General Readings
  1. Hynes G, Celis JE, Lewis VA, Carne A, U S, Lauridsen JB, et al. Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits. Electrophoresis. 1996 Nov;17(11):1720-7. PubMed PMID: 8982604.
  2. Llorca O, Martín-Benito J, Ritco-Vonsovici M, Grantham J, Hynes GM, Willison KR, et al. Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations. EMBO J. 2000 Nov 15;19(22):5971-9. PubMed PMID: 11080144. (Free PMC Article available, 6 images available)
  3. Hynes GM, Willison KR. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. J Biol Chem. 2000 Jun 23;275(25):18985-94. PubMed PMID: 10748209.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Aff - Purified
Specificity
Specificity:
This antibody recognises the theta polypeptide of the CCT chaperonin molecule complex.
Species:
Bovine, Rabbit, Human and Mouse.
Gene ID 10694
FocusOn and Reviews
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Accessory Products

Proteins and/or Positive Controls

Proteins for CCT8 / TCP1 theta (1 products)

Catalog No. Species Pres. Purity   Source  

CCT8 / TCP1 theta

CCT8 / TCP1 theta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / $680.00

Special Price: 20 µg / $398.00

  OriGene Technologies, Inc.

Positive controls for CCT8 / TCP1 theta (1 products)

Catalog No. Species Pres. Purity   Source  

CCT8 overexpression lysate

CCT8 overexpression lysate
0.1 mg / $280.00
  OriGene Technologies, Inc.
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