discontinued

AP16211PU-N CCT1 / TCP1 (C-term) antibody

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Goat anti Human CCT1 / TCP1

AP16211PU-N

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  • AP16211PU-N

Product Description for CCT1 / TCP1

Goat anti Human CCT1 / TCP1.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for CCT1 / TCP1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CCT alpha, CCTA, Chaperonin containing T complex polypeptide 1 subunit 1, T-complex protein 1 subunit alpha, TCP-1-alpha, TCP1 alpha
Presentation Aff - Purified
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17987
Immunogen:
Peptide from C Terminus of the protein sequence according to NP_110379.2; NP_001008897.1.
AA Sequence:
C-SYEDAVHSGALND
GeneID:
6950
Property C-term
Application Peptide ELISA: 1/32000 (Detection Limit).
Western blot: 0.5-2 µg/ml. Approx 60kDa band observed in Hela, Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2).
Immunohistochemistry on Paraffin Sections: 1-2 µg/ml.
In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli.
Background TCP1 alpha is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
Concentration 0.5 mg/ml
General Readings Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature. 1992 Jul 16;358(6383):245-8. PMID: 1630491
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects CCT1 / TCP1 at C-term. It is expected to recognise both reported isoforms.
Gene ID 6950
FocusOn and Reviews
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Accessory Products

Proteins and/or Positive Controls

Proteins for CCT1 / TCP1 (3 products)

Catalog No. Species Pres. Purity   Source  

CCT1 / TCP1 (transcript variant 1)

CCT1 / TCP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $680.00
  OriGene Technologies, Inc.

CCT1 / TCP1 (1-556, His-tag)

CCT1 / TCP1 Human Purified > 80 % E. coli
0.25 mg / $790.00
  Acris Antibodies GmbH

CCT1 / TCP1 (1-556, His-tag)

CCT1 / TCP1 Human Purified > 80 % E. coli
50 µg / $340.00
  Acris Antibodies GmbH

Positive controls for CCT1 / TCP1 (1 products)

Catalog No. Species Pres. Purity   Source  

TCP1 overexpression lysate

TCP1 overexpression lysate
0.1 mg / $280.00
  OriGene Technologies, Inc.
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