TA309309 CACNA1H antibody

Mouse Monoclonal anti-CACNA1H Antibody

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0.1 mg / $410.00
Delivery Time: 2-3 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Human CACNA1H S55-10

Product Description for CACNA1H

Mouse anti Human CACNA1H S55-10.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for CACNA1H

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Cav3.2, Low-voltage-activated calcium channel alpha1 3.2 subunit, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2
Presentation Purified
Reactivity Hu
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone S55-10
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O95180
Immunogen:
Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2 (also known as voltagedependent T-type calcium channel subunit alpha-1H
GeneID:
8912
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western blot: 1 - 10 µg/ml (if results are off, try using the lysate without boiling).
Immunoprecipitation.
Immunocytochemistry: 0.1 - 1.0 µg/ml (Perox).
Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics.
The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
Specifically, Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (3, 4). Studies also suggest that the up-regulations of Cav3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (5).
Concentration 1.0 mg/ml
General Readings
  1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA.
  2. www.iochannels.org
  3. Chen Y., et al. (2003) Ann. Neurol. 54(2): 239–43.
  4. Khosravani H., et al. (2004) J Biol Chem. 279(11): 9681-9684.
  5. Gackiere F., et al. (2008) J Biol Chem. 283(28): 19872.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G chromatography
Buffer System:
PBS pH7.4, 50% glycerol and 0.09% sodium azide
State:
Liquid Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody detects Cav3.2 Ca2+ Channel at ~260kDa. No cross-reactivity against Cav1.3.

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