SM1667FS Bromodeoxyuridine / BrDU antibody

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Rat anti Bromodeoxyuridine / BrDU BU1/75 (ICR1)


Product Description for Bromodeoxyuridine / BrDU

Rat anti Bromodeoxyuridine / BrDU BU1/75 (ICR1).
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Bromodeoxyuridine / BrDU

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Presentation FITC
Applications F, ICC/IF
Clonality Monoclonal
Clone BU1/75 (ICR1)
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Immunocytochemistry: 1/50-1/250.
Background The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Concentration 0.5 mg/ml
General Readings
  1. Perez-Ruiz A et al. {beta}-catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci : (2008).
  2. Iulianella A, Sharma M, Durnin M, Vanden Heuvel GB, Trainor PA. Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis. Development. 2008 Feb;135(4):729-41. doi: 10.1242/dev.013276. PubMed PMID: 18223201. (Free PMC Article available, 9 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
0.09% Sodium Azide
50% Glycerol
Liquid purified IgG fraction
Reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. It also reacts with chlorodeoxyuridine but with reduced staining.
The antibody does not cross react with Thymidine.

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