DDX0910-HRPO-100 Alpha-Synuclein / SNCA antibody

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0.1 mg / $490.00
Delivery Time: 7-10 working days*
(*) valid for US customers only

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Mouse anti Human Alpha-Synuclein / SNCA AS11

DDX0910-HRPO-100

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  • DDX0910-HRPO-100

Product Description for Alpha-Synuclein / SNCA

Mouse anti Human Alpha-Synuclein / SNCA AS11.
Presentation: HRP
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Alpha-Synuclein / SNCA

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, PARK1
Presentation HRP
Reactivity Hu
Applications C, WB
Clonality Monoclonal
Clone AS11
Host Mouse
Isotype IgG
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P37840
Immunogen:
rhu α-synuclein (SIGMA).
GeneID:
6622
Application DDX0910P-50 / DDX0910P-100 Purified: ImmunoFluorescence, ImmunoHistoChemistry frozen sections, Western Blot. 
DDX0910A488-50 / DDX0910A488-100 Alexa-fluor®488: ImmunoFluorescence. 
DDX0910A546-50 / DDX0910A546-100 Alexa-fluor®546: ImmunoFluorescence.  
DDX0910A647-50 / DDX0910A647-100 Alexa-fluor®647: ImmunoFluorescence. 
DDX0910HRP-50 / DDX0910HRP-100 Horseradish Peroxydase: ImmunoHistoChemistry frozen sections, Western Blot. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background α-synuclein (snca) is a140 aas neuronal protein predominantly present in synaptic junctions, specially studied for its relevance in understanding sporadic and familial forms of Parkinson’s disease and related neurodegenerative disorders. In an attempt to break the immunotolerance since the amino-acid sequence is highly conserved between species (95.3% homology between human and mouse), we have immunized C57BL/6 inbred mice presenting a natural deletion for snca with recombinant human α-synuclein and obtained a murine mAb (AS11) recognizing endogenous human α-synuclein. (Kahle et al.,2000, J Neurosci 2000;20:6365–73 ; Specht and Schoepfer, BMC Neurosci 2001;2:11 ; Mougenot AL et al, J Neurosci Methods. 2010 Oct 15;192(2):268-76).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Mougenot AL, Bétemps D, Hogeveen KN, Kovacs GG, Chouaf-Lakhdar L, Milhavet O, et al. Production of a monoclonal antibody, against human α-synuclein, in a subpopulation of C57BL/6J mice, presenting a deletion of the α-synuclein locus. J Neurosci Methods. 2010 Oct 15;192(2):268-76. doi: 10.1016/j.jneumeth.2010.08.010. Epub 2010 Aug 13. PubMed PMID: 20709102.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
HRP
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
Specificity
Specificity:
N-terminal portion of Human α-synuclein (aa1-60)
Species cross-reactivity: Negative for Murine α-synuclein.

Accessory Products

Proteins and/or Positive Controls

Proteins for Alpha-Synuclein / SNCA (29 products)

Catalog No. Species Pres. Purity   Source  

Alpha-Synuclein / SNCA (transcript variant NACP140)

Alpha-Synuclein / SNCA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA (transcript variant NACP112)

Alpha-Synuclein / SNCA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA (transcript variant 2)

Alpha-Synuclein / SNCA Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA (transcript variant 3)

Alpha-Synuclein / SNCA Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA (full length, transcript variant 1)

Alpha-Synuclein / SNCA Human > 95 %
Preparation: .
Purity Detail: > 95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
0.1 mg / $411.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA (A30P Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (A30P Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-140)

Alpha-Synuclein / SNCA Human Purified > 95 % pure by SDS-PAGE. E. coli
0.1 mg / $240.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-140)

Alpha-Synuclein / SNCA Human Purified > 95 % E. coli
0.1 mg / $240.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-140)

Alpha-Synuclein / SNCA Human Purified > 95 % pure by SDS-PAGE. E. coli
1 mg / $570.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-140)

Alpha-Synuclein / SNCA Human Purified > 95 % E. coli
1 mg / $570.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (A53T Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (A53T Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (A30P/A53T Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (A30P/A53T Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (Delta-Nac Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (Delta-Nac Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (NACP112)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (NACP112)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (61-140)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (61-140)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE. E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (96-140)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (96-140)

Alpha-Synuclein / SNCA Human Purified > 95 % E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-60)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-60)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (E46K Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (E46K Mutant)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-95)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / $250.00
  OriGene Technologies GmbH

Alpha-Synuclein / SNCA (1-95)

Alpha-Synuclein / SNCA Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / $600.00
  OriGene Technologies GmbH

Positive controls for Alpha-Synuclein / SNCA (3 products)

Catalog No. Species Pres. Purity   Source  

SNCA overexpression lysate

SNCA overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

SNCA overexpression lysate

SNCA overexpression lysate
0.1 mg / $315.00
  OriGene Technologies, Inc.

SNCA overexpression lysate

SNCA overexpression lysate
0.1 mg / $315.00
  OriGene Technologies, Inc.
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