TA320088 ALKBH2 / ABH2 (C-term) antibody

Rabbit Polyclonal ALKBH2 Antibody

See related secondary antibodies

Search for all "ALKBH2 / ABH2"

0.1 mg / $415.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human, Mouse, Rat ALKBH2 / ABH2

TA320088

More Views

  • TA320088

Product Description for ALKBH2 / ABH2

Rabbit anti Human, Mouse, Rat ALKBH2 / ABH2.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for ALKBH2 / ABH2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Oxy DC1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6NS38
Immunogen:
15 amino acid peptide near the carboxy terminus of human ALKBH2
GeneID:
121642
Property C-term
Application ELISA.
Western blot: 1 - 2 µg/ml.
Positive control: Human Kidney Tissue Lysate.
Background The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.
Concentration 1.0 mg/ml
General Readings
  1. Duncan T, Trewick SC, Koivisto P, Bates PA, Lindahl T, Sedgwick B. Reversal of DNA alkylation damage by two human dioxygenases. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16660-5. Epub 2002 Dec 16. PubMed PMID: 12486230. (Free PMC Article available, 5 images available)
  2. Cetica V, Genitori L, Giunti L, Sanzo M, Bernini G, Massimino M, et al. Pediatric brain tumors: mutations of two dioxygenases (hABH2 and hABH3) that directly repair alkylation damage. J Neurooncol. 2009 Sep;94(2):195-201. doi: 10.1007/s11060-009-9837-0. Epub 2009 Mar 17. PubMed PMID: 19290481.
  3. Chen H, Giri NC, Zhang R, Yamane K, Zhang Y, Maroney M, et al. Nickel ions inhibit histone demethylase JMJD1A and DNA repair enzyme ABH2 by replacing the ferrous iron in the catalytic centers. J Biol Chem. 2010 Mar 5;285(10):7374-83. doi: 10.1074/jbc.M109.058503. Epub 2009 Dec 30. PubMed PMID: 20042601. (Free PMC Article available, 7 images available)
  4. Lee SY, Luk SK, Chuang CP, Yip SP, To SS, Yung YM. TP53 regulates human AlkB homologue 2 expression in glioma resistance to Photofrin-mediated photodynamic therapy. Br J Cancer. 2010 Jul 27;103(3):362-9. doi: 10.1038/sj.bjc.6605797. PubMed PMID: 20661249. (Free PMC Article available, 4 images available)
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% sodium azide
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, mouse, rat

Accessory Products

Proteins and/or Positive Controls

Proteins for ALKBH2 / ABH2 (5 products)

Catalog No. Species Pres. Purity   Source  

ALKBH2 / ABH2 (transcript variant 2)

ALKBH2 / ABH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

ALKBH2 / ABH2 (transcript variant 1)

ALKBH2 / ABH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

ALKBH2 / ABH2 (transcript variant 3)

ALKBH2 / ABH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

ALKBH2 / ABH2 (1-261, His-tag)

ALKBH2 / ABH2 Human Purified > 90 % E. coli
0.5 mg / $820.00
  OriGene Technologies GmbH

ALKBH2 / ABH2 (1-261, His-tag)

ALKBH2 / ABH2 Human Purified > 90 % E. coli
0.1 mg / $320.00
  OriGene Technologies GmbH

Positive controls for ALKBH2 / ABH2 (3 products)

Catalog No. Species Pres. Purity   Source  

ALKBH2 overexpression lysate

ALKBH2 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

ALKBH2 overexpression lysate

ALKBH2 overexpression lysate
0.1 mg / $315.00
  OriGene Technologies, Inc.

ALKBH2 overexpression lysate

ALKBH2 overexpression lysate
0.1 mg / $315.00
  OriGene Technologies, Inc.
  • LinkedIn