AP09101HR-S Alkaline phosphatase / ALPI / IAP antibody

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0.1 mg / $515.00
Delivery Time: 2-3 working days*
(*) valid for US customers only

Quick Overview

Sheep anti Bovine Alkaline phosphatase / ALPI / IAP

AP09101HR-S

Product Description for Alkaline phosphatase / ALPI / IAP

Sheep anti Bovine Alkaline phosphatase / ALPI / IAP.
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Alkaline phosphatase / ALPI / IAP

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Intestinal alkaline phosphatase
Presentation HRP
Reactivity Bov
Applications E, WB
Clonality Polyclonal
Host Sheep
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P19111
Immunogen:
Alkaline phosphatase from calf intestine
Application Western blot: 1/500-1/2000.
ELISA: 1/4000 to 1/20000.
Background Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Caution Do NOT add Sodium azide!
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish peroxidase
HRP
Purification:
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Stabilizers:
10 mg/ml BSA (immunoglobulin and protease free)
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
For extended storage reconstitute product with 50% glycerol instead of water.
Specificity
Specificity:
This antibody reacts to bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined.
Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified alkaline phosphatase [calf intestine].
Species:
Bovine.

Accessory Products

Proteins and/or Positive Controls

Proteins for Alkaline phosphatase / ALPI / IAP (4 products)

Catalog No. Species Pres. Purity   Source  

Alkaline phosphatase / ALPI / IAP

Alkaline phosphatase / ALPI / IAP Bovine Purified 95.0% pure by Gel Filtration.
  OriGene Technologies GmbH

Alkaline phosphatase / ALPI / IAP

Alkaline phosphatase / ALPI / IAP Bovine Purified 95.0% pure by Gel Filtration.
  OriGene Technologies GmbH

Alkaline phosphatase / ALPI / IAP

Alkaline phosphatase / ALPI / IAP Bovine Purified 95.0% pure by Gel Filtration.
  OriGene Technologies GmbH

Alkaline phosphatase / ALPI / IAP

Alkaline phosphatase / ALPI / IAP Bovine Intestine
5 mg / $300.00
  OriGene Technologies GmbH
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