TA319292 Albumin antibody

Rabbit polyclonal Human Serum Albumin antibody Peroxidase Conjugated

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1 mg / $1,345.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Human Albumin

Product Description for Albumin

Rabbit anti Human Albumin.
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Albumin

Product Category Primary Antibodies
Target Category
Quantity 1 mg
Synonyms ALB, BSA, HSA, Serum Albumin
Presentation HRP
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P02768
Immunogen:
Human Serum Albumin.
GeneID:
213
Application Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Note: This product has been assayed against 1.0 µg of Human Albumin IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature.
A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested for this product.
Background Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Caution Do Not Add Sodium Azide.
Concentration 1.0 mg/ml (before lyophilization by UV absorbance at 280 nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated below.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Gentamicin Sulfate as preservative and Polyethylen Glycol (PEG-8000) as stabilizer.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified human serum albumin IgG, and human Serum.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Albumin (25 products)

Catalog No. Species Pres. Purity   Source  

Albumin

Albumin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Albumin

Albumin Human Purified > 99.98% as determined by both RP-HPLC and SDS-PAGE
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99.98% as determined by both RP-HPLC and SDS-PAGE
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99.98% as determined by both RP-HPLC and SDS-PAGE
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 98.0% as determined by both RP-HPLC and SDS-PAGE analysis
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 98.0% as determined by both RP-HPLC and SDS-PAGE analysis
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 98.0% as determined by both RP-HPLC and SDS-PAGE analysis
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99.0% as determined by both RP-HPLC and SDS-PAGE analysis
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99.0% as determined by both RP-HPLC and SDS-PAGE analysis
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99.0% as determined by both RP-HPLC and SDS-PAGE analysis
  OriGene Technologies GmbH

Albumin

Albumin Human > 99 % Serum
  OriGene Technologies GmbH

Albumin

Albumin Human Purified This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc.
Serum
  OriGene Technologies GmbH

Albumin (19-609, His-tag)

Albumin Human Purified > 90 % by SDS - PAGE.
0.25 mg / $1,070.00
  OriGene Technologies GmbH

Albumin (19-609, His-tag)

Albumin Human Purified > 90 % by SDS - PAGE.
50 µg / $320.00
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity is greater than 98.% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Mammalian cells
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity is greater than 98.% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Mammalian cells
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity is greater than 98.% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Mammalian cells
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Serum
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Serum
  OriGene Technologies GmbH

Albumin

Albumin Human Biotin This product was prepared from normal serum delipidation, a multi-stage process including selective precipitation. Serum
1 mg / $205.00
  OriGene Technologies GmbH

Albumin

Albumin Human FITC This product was prepared from chromatographically pure Human Albumin. Serum
1 mg / $175.00
  OriGene Technologies GmbH

Albumin

Albumin Human HRP This product was prepared from normal serum by a multi-step process including selective precipitaition and tandem chromatography followed by extensive dialysis against the buffer stated above. Serum
1 mg / $210.00
  OriGene Technologies GmbH

Albumin

Albumin Human TRITC This product was prepared from chromatographically pure Human Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin. Serum
1 mg / $175.00
  OriGene Technologies GmbH

Albumin

Albumin Human Texas Red This product was prepared from normal serum by a multi-step process including selective precipitation and extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum.
Serum
1 mg / $205.00
  OriGene Technologies GmbH

Albumin

Albumin Human Purified > 99 % Urine
  BBI Solutions

Positive controls for Albumin (1 products)

Catalog No. Species Pres. Purity   Source  

ALB overexpression lysate

ALB overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.
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