TA319560 AKT1/AKT2/AKT3 antibody

Mouse monoclonal anti-AKT antibody

See related secondary antibodies

Search for all "AKT1/AKT2/AKT3"

0.1 mg / $515.00
Delivery Time: 2-3 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Chimpanzee, Human, Mouse, Rat AKT1/AKT2/AKT3 14E5.16C8.25F6

Product Description for AKT1/AKT2/AKT3

Mouse anti Chimpanzee, Human, Mouse, Rat AKT1/AKT2/AKT3 14E5.16C8.25F6.
Presentation: Purified
Product is tested for Immunoprecipitation, Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for AKT1/AKT2/AKT3

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms AKT1, AKT2, AKT3, PKB, PKB alpha, PKB beta, PKB gamma, PKBG, Protein kinase Akt-2, Protein kinase Akt-3, Protein kinase B, Protein kinase B alpha, Protein kinase B beta, Protein kinase B gamma, Proto-oncogene c-Akt, RAC, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine-protein kinase, STK-2
Presentation Purified
Reactivity Chimp, Hu, Ms, Rt
Applications C, E, IP, WB
Clonality Monoclonal
Clone 14E5.16C8.25F6
Host Mouse
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P31749
Immunogen:
Synthetic peptide corresponding to residues near the C terminal end of of Human AKT1 protein
GeneID:
207
Application ELISA: 1/20,000.
Western Blot: 1/500-1/3,000. Expect a band approximately 54-56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. 
Immunoprecipitation.
Immunohistochemistry: 20 µg/ml. We recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Lawlor MA, Alessi DR. PKB/Akt: a key mediator of cell proliferation, survival and insulin responses? J Cell Sci. 2001 Aug;114(Pt 16):2903-10. PubMed PMID: 11686294.
  2. Alessi DR. Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture. Biochem Soc Trans. 2001 May;29(Pt 2):1-14. PubMed PMID: 11356119.
  3. Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA. Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4171-5. PubMed PMID: 1851997. (Free PMC Article available)
  4. Staal SP. Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma. Proc Natl Acad Sci U S A. 1987 Jul;84(14):5034-7. PubMed PMID: 3037531. (Free PMC Article available)
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) purified IgG fraction
Purified
Species Reactivity
Species reactivity (expected):
Rat, Chimpanzee.
Species reactivity (tested):
Human, Mouse.
Specificity
Specificity:
This antibody is specific for Human and Mouse AKT protein.
A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from Human, Mouse, Rat and Chimpanzee sources based on 100% homology with the immunizing sequence. 
Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1/AKT2/AKT3 (23 products)

Catalog No. Species Pres. Purity   Source  

AKT1 / PKB (transcript variant 3)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 2)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (C-term MYC/DDK tag, transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (Substrate)

AKT1 / PKB Human Purified > 98 % pure by HPLC analysis.
0.5 mg / $305.00
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (1-480, His-tag)

AKT1 / PKB Human Purified > 90 % by SDS - PAGE.
0.25 mg / $1,070.00
  OriGene Technologies GmbH

AKT1 / PKB (1-480, His-tag)

AKT1 / PKB Human Purified > 90 % by SDS - PAGE.
50 µg / $320.00
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH
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