AP22624SU-N AKT1/AKT2/AKT3 (C-term) antibody

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50 µl / $425.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

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Rabbit anti Chicken, Human, Mouse, Rat AKT1/AKT2/AKT3

AP22624SU-N

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Product Description for AKT1/AKT2/AKT3

Rabbit anti Chicken, Human, Mouse, Rat AKT1/AKT2/AKT3.
Properties: (C-term)
Presentation: Serum
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for AKT1/AKT2/AKT3

Product Category Primary Antibodies
Target Category
Quantity 50 µl
Synonyms AKT-2, AKT-3 Review 010, Akt-1, C-AKT, PKB gamma, PKBG, Protein kinase B, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma
Presentation Serum
Reactivity Chk, Hu, Ms, Rt
Applications E, ICC/IF, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P31749
Immunogen:
Synthetic peptide -KLH conjugated corresponding to the C-terminus (460-480) of Human, Rat, Mouse and Chicken AKT proteins conjugated to KLH using maleimide.
AA Sequence:
C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A
GeneID:
207
Remarks:
A residue of cysteine was added to the amino terminal end to facilitate coupling.
Property C-term
Application ELISA: 1/2000 - 1/10000.
Immunofluorescence: 1/100 - 1/1000.
Immunohistochemistry on Paraffin Sections: 1/100.
Immunoprecipitation.
Western Blot: 1/500 - 1/2000.
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis and (ii) promotion of proliferation.
Concentration 75 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Delipidation and Defibrination
Buffer System:
0.02 M potassium phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.09% Sodium Azide
State:
Liquid Ig fraction
Serum
Species Reactivity
Species reactivity (expected):
Chicken.
Species reactivity (tested):
Human, Mouse and Rat.
Specificity
Specificity:
Recognizes AKT (AKT1/AKT2/AKT3).
The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.
Gene ID 207

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1/AKT2/AKT3 (23 products)

Catalog No. Species Pres. Purity   Source  

AKT1 / PKB (transcript variant 3)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 2)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (C-term MYC/DDK tag, transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (Substrate)

AKT1 / PKB Human Purified > 98 % pure by HPLC analysis.
0.5 mg / $305.00
  OriGene Technologies GmbH

AKT1 / PKB (1-480, His-tag)

AKT1 / PKB Human Purified > 90 % by SDS - PAGE.
0.25 mg / $1,070.00
  OriGene Technologies GmbH

AKT1 / PKB (1-480, His-tag)

AKT1 / PKB Human Purified > 90 % by SDS - PAGE.
50 µg / $320.00
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH
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