TA337148 AIFM1 / AIF antibody

Rabbit Polyclonal AIF Antibody

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50 µl / $335.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

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Rabbit anti Bovine, Canine, Human, Mouse, Rat AIFM1 / AIF

TA337148

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  • TA337148

Product Description for AIFM1 / AIF

Rabbit anti Bovine, Canine, Human, Mouse, Rat AIFM1 / AIF.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for AIFM1 / AIF

Product Category Primary Antibodies
Target Category
Quantity 50 µl
Synonyms Apoptosis-inducing factor 1 mitochondrial, PDCD8, Programmed cell death protein 8
Presentation Purified
Reactivity Bov, Can, Hu, Ms, Rt
Applications C, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O95831-2
Immunogen:
(aa 151-170); human.
GeneID:
9131
Application WB: 1:1000-1:2000~
IHC-F: 1:1000-1:5000
IHC-P: 1:1000-1:5000~
IP: 1:50-1:200
Background AIF (apoptosis-inducing factor) was initially discovered as a protein involved in caspase-independent cell death. It is now known that AIF has both vital and lethal functions (reviewed in Modjtahedi et al, 2006). In healthy cells, AIF is a flavoprotein present in the mitochondria where it has vital roles in cellular redox metabolism and mitochondrial bioenergetics. In many models of apoptosis, AIF is released from the mitochondria during mitochondrial outer membrane permeabilization along with other mitochondrial proteins. Upon release, AIF translocates first to the cytosol and then to the nucleus where it induces chromatin condensation and D degradation. Although several lines of evidence suggest that AIF is a main mediator of capsase-independent cell death, the mechanisms regulating AIF pro-apoptotic function remain to be fully elucidatied and may depend on the cell type and type of apoptotic stimuli. Human AIF is transcribed from a nuclear gene located on the X chromosome and translated in the cytoplasm to a precursor protein of 613 amino acids (aa) which corresponds to ~67 kDa. The precursor protein is imported into the mitochonria by mitochondrial localization sequences located within the N-termil prodomain of AIF. Once inside the mitochondria, the prodomain is cleaved giving rise to a mature AIF form of ~57 kDa. AIF isoforms generated from a single AIF gene have been identified, including AIF short isoform 2 (324 aa protein; GenBank no. AAY84739.1) and AIF short isoform 3 (237 aa protein; GenBank noAAY84741.1 (reviewed in Delttre et al, 2006). The generation of multiple isoforms from a common gene is an evolutiory mechanism that increases protein diversiity in eukaryotes. Regulating gene expression through the production of multiple isoforms from a single gene is thought to play a major role in the control of apoptosis and other forms of programmed cell death. It recognizes AIF and AIF isoforms containing the peptide immunogen sequence, RARDPGARVLIVSEDPELP.
Format
Purification:
Whole antisera
Buffer System:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
State:
Purified

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