TA326464 AHA1 / AHSA1 antibody

Rat Monoclonal Anti-Aha2 Antibody

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0.1 mg / $426.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

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Rat anti Human, Mouse, Rat AHA1 / AHSA1 25F2.D9

TA326464

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  • TA326464
  • TA326464

Product Description for AHA1 / AHSA1

Rat anti Human, Mouse, Rat AHA1 / AHSA1 25F2.D9.
Presentation: Purified
Product is tested for Paraffin Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for AHA1 / AHSA1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Activator of 90 kDa heat shock protein ATPase homolog 1, C14orf3, HSPC322
Presentation Purified
Reactivity Hu, Ms, Rt
Applications E, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 25F2.D9
Host Rat
Isotype IgG2a
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8BK64
Immunogen:
Mouse Aha1.
GeneID:
217737
Isotype control SM15P, SM15PX
Application ELISA.
Western blot: 
1 μg/mL of this antibody was sufficient for detection of Aha1 in 10μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Immunoprecipitation.
Immunofluorescence.
Immunocytochemistry.
Immunohistochemitry on Paraffin Sections:
5 µg/ml (heat-induced antigen retrieval is required).
Background Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Concentration 1.2 mg/ml
General Readings
  1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
  2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
  3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
  4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
0.02M Potassium Phosphate, pH 7.2 containing 0.15M Sodium Chloride
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse and Rat.
Specificity
Specificity:
This antibody detects ~38kDa, can run at up to 45kD on SDS-PAGE.
1 μg/ml of this antibody was sufficient for detection of Aha1 in 10 μg of Rat tissue lysate by colorimetric Immunoblot analysis using Goat anti-Rat IgG-HRP as the secondary antibody.

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