SM1353P Aggrecan antibody

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0.2 mg / $570.00
Delivery Time: 3-5 working days*
(*) valid for US customers only

Quick Overview

Mouse anti Bovine, Human Aggrecan HAG7D4 (7D4)

Product Description for Aggrecan

Mouse anti Bovine, Human Aggrecan HAG7D4 (7D4).
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Aggrecan

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms ACAN, AGC1, CSPG1, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, MSK16
Presentation Purified
Reactivity Bov, Hu
Applications C, E, IP, P, WB
Clonality Monoclonal
Clone HAG7D4 (7D4)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P16112
Immunogen:
Purified Human articular cartilage Aggrecan.
GeneID:
176
Remarks:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA.
Western blot.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections
: Requires a standard heat mediated antigen retrieval using a microwave.
Histology Positive Control Tissue: Cerebral Cortex.
Background Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Anders JO, Mollenhauer J, Beberhold A, Kinne RW, Venbrocks RA. Gelatin-based haemostyptic Spongostan as a possible three-dimensional scaffold for a chondrocyte matrix?: an experimental study with bovine chondrocytes. J Bone Joint Surg Br. 2009 Mar;91(3):409-16. doi: 10.1302/0301-620X.91B3.20869. PubMed PMID: 19258622.
General Readings
  1. Virgintino D, Perissinotto D, Girolamo F, Mucignat MT, Montanini L, Errede M, et al. Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex. J Cell Mol Med. 2009 Sep;13(9B):3151-73. doi: 10.1111/j.1582-4934.2009.00694.x. Epub 2009 Feb 9. PubMed PMID: 19220578.
  2. Shintani N, Hunziker EB. Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: influence of microenvironment, tissue origin and growth factor. Eur Cell Mater. 2011 Nov 24;22:302-19; discussion 319-20. PubMed PMID: 22116649.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Purified
Species Reactivity
Species reactivity (tested):
Human and Bovine.
Specificity
Specificity:
This antibody Clone 7D4 Cat.-No SM1353P recognizes Human Aggrecan, a proteoglycan component of cartilage matrix and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).
Studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009 in Ref.1). 
SM1353P recognizes epitopes within the N-terminal G1-IGD-G2 region of Aggrecan, that has been identified as a cluster 1 isoform. 
Negative Species: Chicken, Fish and Rat.

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