Product Description for 5-Methyl Cytosine / 5-MeC
Mouse anti Human, Mouse, Rat 5-Methyl Cytosine / 5-MeC 33D3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.
Properties for 5-Methyl Cytosine / 5-MeC
Product Category | Primary Antibodies |
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Target Category | |
Quantity | 0.1 mg |
Synonyms | 5-MeCyd, 5-Methyl Cytidine, 5-Methylcytidine, 5MeC |
Presentation | Purified |
Reactivity | Hu, Ms, Rt |
Applications | C, F, ICC/IF, IP, P, WB |
Clonality | Monoclonal |
Clone | 33D3 |
Host | Mouse |
Isotype | IgG1 |
Shipping to | Worldwide |
PDF datasheet | View Datasheet |
Manufacturer | OriGene Technologies GmbH |
Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
Immunogen |
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Isotype control | AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples) |
Application | Flow Cytometry: Use at an assay dependent dilution. (Use 10 µl of working dilution to label 1,000,000 cells in 100 µl. (See Habib,M. et al. 1999) Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described in Ref.4 (Giraldo, A. M. et al.) Western blot/Immunoblotting: 1/250 (Southern blot 1/200). Immunofluorescence: 1/500. Immunohistochemistry on Frozen and Paraffin Embedded Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Methylated DNA Immunoprecipitation (MeDIP): Use at an assay dependet concentration, 1-2 µg per IP. Immunocytochemistry: 0.5-5 µg/ml. ELISA: 1/10000 OR use at an assay dependent concentration. |
Background | Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when Cytosine is attached to a Ribose ring (also known as a Ribofuranose) via a beta-N1-glycosidic bond. |
Concentration | 1.0 mg/ml (at O.D. 280nm) |
Product Citations |
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Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
Format |
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Species Reactivity |
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Specificity |
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Accessory Products
Recommended Secondary Antibodies for 5-Methyl Cytosine / 5-MeC (11 products)
Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
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R1253AP |
Mouse IgG (H+L chain) antibody |
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Rabbit | AP | C, E, WB | ||||
OriGene Technologies GmbH | |||||||
R1253B |
Mouse IgG (H+L chain) antibody |
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Rabbit | Biotin | C, E, WB | ||||
OriGene Technologies GmbH | |||||||
R1253F |
Mouse IgG (H+L chain) antibody |
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Rabbit | FITC | E, ICC/IF | ||||
OriGene Technologies GmbH | |||||||
R1253HRP |
Mouse IgG (H+L chain) antibody |
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Rabbit | HRP | C, E, WB | ||||
OriGene Technologies GmbH | |||||||
R1253TR |
Mouse IgG (H+L chain) antibody |
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Rabbit | Texas Red | ICC/IF | ||||
OriGene Technologies GmbH | |||||||
R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Rabbit | PE | F | ||||
OriGene Technologies GmbH | |||||||
R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Goat | PE | |||||
OriGene Technologies GmbH | |||||||
R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Donkey | PE | |||||
OriGene Technologies GmbH | |||||||
R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy2 | |||||
OriGene Technologies GmbH | |||||||
R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy3 | |||||
OriGene Technologies GmbH | |||||||
R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy5 | |||||
OriGene Technologies GmbH |