SM1872P 5-Methyl Cytosine / 5-MeC antibody

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Mouse anti Human, Mouse, Rat 5-Methyl Cytosine / 5-MeC 33D3


Product Description for 5-Methyl Cytosine / 5-MeC

Mouse anti Human, Mouse, Rat 5-Methyl Cytosine / 5-MeC 33D3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for 5-Methyl Cytosine / 5-MeC

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 5-MeCyd, 5-Methyl Cytidine, 5-Methylcytidine, 5MeC
Presentation Purified
Reactivity Hu, Ms, Rt
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 33D3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0Ag 14 myeloma cell line
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Flow Cytometry: Use at an assay dependent dilution. (Use 10 µl of working dilution to label 1,000,000 cells in 100 µl. (See Habib,M. et al. 1999)
Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described in Ref.4 (Giraldo, A. M. et al.)
Western blot/Immunoblotting: 1/250 (Southern blot 1/200).
Immunohistochemistry on Frozen and Paraffin Embedded Sections:
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Methylated DNA Immunoprecipitation (MeDIP): Use at an assay dependet concentration1-2 µg per IP.
Immunocytochemistry: 0.5-5 µg/ml.
ELISA: 1/10000 OR use at an assay dependent concentration.
Background Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when Cytosine is attached to a Ribose ring (also known as a Ribofuranose) via a beta-N1-glycosidic bond.
Concentration 1.0 mg/ml (at O.D. 280nm)
Product Citations
Originator or purchased from resellers:
  1. Reynaud C, Bruno C, Boullanger P, Grange J, Barbesti S, Niveleau A. Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 1992 Jan 31;61(3):255-62. PubMed PMID: 1739950.
  2. Habib M, Fares F, Bourgeois CA, Bella C, Bernardino J, Hernandez-Blazquez F, et al. DNA global hypomethylation in EBV-transformed interphase nuclei. Exp Cell Res. 1999 May 25;249(1):46-53. PubMed PMID: 10328952.
  3. Hernandez-Blazquez FJ, Habib M, Dumollard JM, Barthelemy C, Benchaib M, de Capoa A, et al. Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 2000 Nov;47(5):689-93. PubMed PMID: 11034586. (Free PMC Article available, 3 images available)
  4. Giraldo AM, Lynn JW, Purpera MN, Godke RA, Bondioli KR. DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Mol Reprod Dev. 2007 Dec;74(12):1514-24. PubMed PMID: 17440941.
  5. Shen R, Tao L, Xu Y, Chang S, Van Brocklyn J, Gao JX. Reversibility of aberrant global DNA and estrogen receptor-alpha gene methylation distinguishes colorectal precancer from cancer. Int J Clin Exp Pathol. 2009;2(1):21-33. Epub 2008 Apr 20. PubMed PMID: 18830381. (Free PMC Article available, 6 images available)
  6. Pontes O, Lawrence RJ, Silva M, Preuss S, Costa-Nunes P, Earley K, et al. Postembryonic establishment of megabase-scale gene silencing in nucleolar dominance. PLoS One. 2007 Nov 7;2(11):e1157. PubMed PMID: 17987131. (Free PMC Article available, 5 images available)
  7. Yang F, Zhang L, Li J, Huang J, Wen R, Ma L, et al. Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize. BMC Plant Biol. 2010 Aug 18;10:178. doi: 10.1186/1471-2229-10-178. PubMed PMID: 20718950. (Free PMC Article available, 7 images available)
  8. Suter JD, Howard DJ, Shi H, Caldwell CW, Fan X. Label-free DNA methylation analysis using opto-fluidic ring resonators. Biosens Bioelectron. 2010 Nov 15;26(3):1016-20. doi: 10.1016/j.bios.2010.08.050. Epub 2010 Aug 20. PubMed PMID: 20846848.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein A
Buffer System:
10 mM PBS, pH 7.4
0.01% Thimerosal
Liquid purified IgG fraction (<98% Gel electophoresis SDS PAGE)
Species Reactivity
Species reactivity (tested):
Human, Rat and Mouse.
This antibody recognizes the modified base 5-Methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNAmethylation is a DNA modification process, which is involved in the control of gene expression.
Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart Cytosine.
Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.

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