AM50445PU-N 2,2,7-Trimethylguanosine antibody

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Mouse anti Human, Mouse 2,2,7-Trimethylguanosine K121

Product Description for 2,2,7-Trimethylguanosine

Mouse anti Human, Mouse 2,2,7-Trimethylguanosine K121.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for 2,2,7-Trimethylguanosine

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Ms
Applications ICC/IF, IP
Clonality Monoclonal
Clone K121
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoprecipitation: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).
Immunocytochemistry: A 1/500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa.and A431 cells.
Immunoaffinity Purification: A representative lot from an independent laboratory was used in IAP (Krainer, A.R., et al. (1988). Nucleic Acids Res. 16(20):9415-9429.).
Background The 2,2,7-trimethylguanosine cap structure is found on the 5’ end of small nuclear RNAs and small nucleolar RNAs. Initially, these RNA molecules are capped with 7-monomethylguanosine (m7G) which is then methylated by the trimethylguanosine synthase enzyme to form 2,2,7-trimethylguanosine. The synthesis of 2,2,7-trimethylguanosine occurs in the cytoplasm and is an important marker for nuclear localization of RNA molecules. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5′ exoribonuclease enzyme. 2,2,7-trimethylguanosine capping may play a role in the expression of viral RNAs.
Concentration lot-specific
Storage Store undiluted at 2-8°C. 
Shelf life: one year from despatch.
Protein G Chromatography
Buffer System:
0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Liquid purified Ig fraction
Demonstrated to react with Human and Mouse.

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