AP20192PU-N 14-3-3 protein zeta/delta antibody

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0.1 mg / $370.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Rabbit anti Human, Mouse, Rat 14-3-3 protein zeta/delta

AP20192PU-N

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  • AP20192PU-N

Product Description for 14-3-3 protein zeta/delta

Rabbit anti Human, Mouse, Rat 14-3-3 protein zeta/delta.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for 14-3-3 protein zeta/delta

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, IP, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 28 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P63104
Immunogen:
Synthetic peptide, corresponding to amino acids 20-70 of Human 14-3-3 ζ.
GeneID:
7534
Application Western blot: 1/500-1/1000.
Immunopreciptaion: 1/50-1/200.
Immunofluorescence: 1/50-1/200. 
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Background 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Concentration 1.0 mg/ml
General Readings
  1. Alsterfjord M, Sehnke PC, Arkell A, Larsson H, Svennelid F, Rosenquist M, et al. Plasma membrane H(+)-ATPase and 14-3-3 isoforms of Arabidopsis leaves: evidence for isoform specificity in the 14-3-3/H(+)-ATPase interaction. Plant Cell Physiol. 2004 Sep;45(9):1202-10. PubMed PMID: 15509843.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific immunogen
Buffer System:
Phosphate Buffered Saline (PBS), pH~7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of 14-3-3 zeta protein.
(region surrounding Val52)
Gene ID 7534

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein zeta/delta (7 products)

Catalog No. Species Pres. Purity   Source  

14-3-3 protein zeta/delta (transcript variant 1)

14-3-3 protein zeta/delta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta (transcript variant 3)

14-3-3 protein zeta/delta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta (transcript variant 4)

14-3-3 protein zeta/delta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta (transcript variant 5)

14-3-3 protein zeta/delta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta (transcript variant 6)

14-3-3 protein zeta/delta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta (1-245, His-tag)

14-3-3 protein zeta/delta Human Purified ≥ 95 by SDS PAGE E. coli
0.5 mg / $820.00
  OriGene Technologies GmbH

14-3-3 protein zeta/delta (1-245, His-tag)

14-3-3 protein zeta/delta Human Purified ≥ 95 by SDS PAGE E. coli
0.1 mg / $320.00
  OriGene Technologies GmbH
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